C-reactive protein and medium weight molecules concentration in serum of rats under conditions of carrageenan-induced inflammation and prolonged prophylactic administration of chondroitin sulfate

O. Blokhina, L. Kot, Ie. Torgalo, K. Dvorshchenko
Taras Shevchenkа National University of Kyiv, Kyiv; Taras Shevchenkа National University of Kyiv, Kyiv; Taras Shevchenkа National University of Kyiv, Kyiv; Taras Shevchenkа National University of Kyiv, Kyiv


The aim of the work was to investigate the preventive effect of Chondroitin Sulfate on the concentration of C-reactive protein and medium weight molecules in blood serum of rats at local inflammation of the hind limb. The studies were conducted on white non-linear, sexually mature male rats weighing 180–240 g, in compliance with the general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – control: animals sub-planar injected 0.1 ml of 0.9 % NaCl solution into the posterior right limb. The second group – animals received a therapeutic dose of 3 mg x kg-1 chondroitin sulfate daily for 28 days daily. The third group – animals were infused intramuscularly with 0.1 ml of 0.9 % NaCl solution in the posterior right limb for 28 days and for 29 days inflammatory edema of the limb was stimulated (animals were sub-planar injected with 0.1 ml of 1 % carrageenan solution to the posterior right limb ) The fourth group – for 28 days rats were daily intramuscularly injected with a therapeutic dose of 3 mg x kg-1 chondroitin sulfate, after which on 29th day, inflammatory edema of the limb was stimulated. Animals were killed 3 hours after injection of carrageenan solution according to the protocol of the ethical committee, and then blood sampling for further research was quickly taken. The total number of animals involved in experimental studies was 40 individuals. Creactive protein concentration was determined by turbidimetric method. The content of medium weight molecules was determined by screening method. It has been established that with carrageenan-induced inflammation of the posterior limb, the concentration of C-reactive protein and medium weight molecules increases in the serum. It was shown that the prophylactic administration of chondroitin sulfate based drug on animals with carrageenan-induced inflammation restored the abovementioned parameters.


acute inflammation of the limb, chondroitin sulfate, C-reactive protein, medium weight molecules

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Gabrielyan N.I. Skriningovyiy metod opredeleniya srednih molekul v biologicheskih zhidkostyah: Metodicheskie rekomendatsii. Mir, c. 11. (1985).

Golovach I. Yu. Osteoartrit: fundamentalnyie i prikladnyie aspekty etiopatogeneza zabolevaniya. Nichego ne stoit na meste // Ukrainskiy revmatologIchnyi zhurnal. – 2014. – No 2 (56). – S. 4-11.

Dobrotina N. A., Kopyitova T. V. Endointoksikatsiya organizma cheloveka: metodologicheskie i metodicheskie aspekty // Uchebnoe posobie. Nizhegorodskiy gosudarstvennyiy universitet im. N. I. Lobachevskogo. – Nizhniy Novgorod, 2004. – S. 72.

Karyakina E. V. Molekuly sredney massyikak integralnyiy pokazatel metabolicheskih narusheniy (obzor literatury) / E. V. Karyakina, S. V. Belova // Klin. labor. diagn. – 2004. – # 3. – S. 3–8.

Kelina N. Yu., Bezruchko N. V., Rubtsov G. K. Biohimicheskie proyavleniya endotoksikoza: metodicheskie aspekty izucheniya i otsenki, prognosticheskaya znachimost (analiticheskiy obzor) // Vest. Tyumen. gosud. un–ta. – 2012. – # 6. – S. 143–147.

Smiyan S. I., Masuk O. M., Slaba U. S. Sindrom endogennoi Intoksikatsii yak marker zapalnogo protsesu pru revmatuchnuh zahvoryuvannyah suglobiv // Visnuk nauk. doslidzhen. – 2000. – No 1. – S. 40–42.

Turbidimetriya v laboratornoy praktike / V. V. Dolgov i dr. – M.: Reafarm. – 2007. – 176 s.

Ammitzboll C. G., Steffensen R., Bogsted M. et al. CRP genotype and haplotype associations with serum C-reactive protein level and DAS28 in untreated early rheumatoid arthritis patients // Arthritis Res Ther. – 2014; 16(5): 475.

Attur M, Krasnokutsky-Samuels S., Samuels J., Steven B. Abramson S. B. Prognostic biomarkers in osteoarthritis // Curr Opin Rheumatol. 2013; 25(1): 136–144.

Bishnoi M., Jain A., Hurkat P., Jain S.K. Chondroitin sulphate: a focus on osteoarthritis // Glycoconj. J. – 2016. – Vol. 33, № 5. – P. 693–705. Available from: http://www.ncbi.nlm.nih.gov.

Campo G. M., Avenoso A., Campo S., Ferlazzo A.M. and Calatroni A. Chondroitin Sulphate: Antioxidant Properties and Beneficial Effects // MiniReviews in Medicinal Chemistry, 2006, 6, 1311-1320.

Campo G.M., Avenoso A., Campo S. et al. The antioxidant activity of chondroitin-4-sulphate, in carbon tetrachloride-induced acute hepatitis in mice, involves NF-κB and caspase activation // Br J Pharmacol. 2008 Nov; 155(6): 945–956.

Chu C.R. Defining Pre-Osteoarthritis Is Key to Prevention // Cartilage. 2016 Apr; 7(2): 204. doi: 10.1177/1947603515618758.

Fehrenbacher J.C., Vasko M.R., Duarte D.B. Models of Inflammation: Carrageenan- or Complete Freund's Adjuvant-Induced Edema and Hypersensitivity in the Rat // Curr Protoc Pharmacol. 2012 Mar; 0 5: Unit5.4. doi: 10.1002/0471141755.ph0504s56

Hillström A., Bylin J., Ragnvi Hagman R. et al. Measurement of serum C-reactive protein concentration for discriminating between suppurative arthritis and osteoarthritis in dogs // BMC Vet Res. 2016; 12: 240.

Michael J. W.-P., Schlüter-Brust K.U., Eysel P. The Epidemiology, Etiology, Diagnosis, and Treatment of Osteoarthritis of the Knee // Dtsch Arztebl Int. 2010 Mar; 107(9): 152–162.

Morris C. J. Carrageenan-induced paw edema in the rat and mouse // Methods Mol. Biol. – 2003. – Vol. 225. – P. 115–121. Available from: http://link.springer.com/protocol/10.1385/1-59259-374-7%3A115.

Protein measurement with Folin phenol reagent / O.H. Lowry et al. // J. Biol. Chem. – 1951. – Vol. 193, № 1. – P. 265–275.

Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and meta-analysis / L. Simon [et al.] // Clin. Infect. Dis. – 2004. – Vol. 39, № 2. – P. 206–217.

Souich P., García A.G., Vergés J., Montell E. Immunomodulatory and anti-inflammatory effects of chondroitin sulphate // J Cell Mol Med. 2009 Aug; 13(8a): 1451–1463.

Sproston N.R., Ashworth J.J. Role of C-Reactive Protein at Sites of Inflammation and Infection // Front Immunol. 2018; 9: 754.

Woolf A.D. Global burden of osteoarthritis and musculoskeletal diseases // BMC Musculoskelet Disord. 2015; 16(Suppl 1): S3. Published online 2015 Dec 1. doi: 10.1186/1471-2474-16-S1-S3.

Zhang J. Meta-analysis of serum C-reactive protein and cartilage oligomeric matrix protein levels as biomarkers for clinical knee osteoarthritis // BMC Musculoskelet Disord. 2018; 19: 22.

Received: 04.03.2019

Revised: 05.04.2019

Signed for the press: 05.04.2019

DOI: http://dx.doi.org/10.17721/1728_2624.2019.26.17-21


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