Proangiogenic effect of newly synthesized compounds-analogue of inhibitors HIF-1

T. Nikolaienko, L. Garmanchuk, S. Orysyk, V. Pekhnyo
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; V.I. Vernadskii Institute of General and Inorganic Chemistry NAS of Ukraine, Kyiv; V.I. Vernadskii Institute of General and Inorganic Chemistry NAS of Ukraine, Kyiv


The effect of new compounds analog of inhibitors of HIF-1 on the metabolic and proliferative status of endothelial cells have been studied. It was shown that syn-isomer possesses of proproliferative effect and antiapoptotic effect with anti-isomer. These effects are consistent with the rates of glucose uptake and production of nitric oxide.


anti-, syn-isomers, glucose, nitric oxide, endothelial cells

Full Text:



Lim C, Kiriakidis S, Sandison А, et al. Hypoxia-inducible factor pathway and diseases of the vascular wall. J Vasc Surg.2013; 8(1):219-30.

Carmeliet P, Jain R. Angiogenesis in cancer and other disease. Nature. 2000; 407:249-57.

Semenza GL,Carmeliet P. HIF-1 and mechanisms of hypoxia sensing. Curr Opin Cell Biol. 2001;1 3:167–71.

Denko NC. Hypoxia, HIF1 and glucose metabolism in the solid tumour. Nat Rev Cancer. 2008; 8:705–13.

Conway EM, Collen D, Carmeliet P. Molecular mechanisms of blood vessel growth . Cardiovasc Res. 2001; 49:507–21.

GreijerAE, Groep P, Kemming D, et al. Upregualtion of gene expression by hypoxia is mediated predominantly by hypoxia-inducible factor I (HIF-I). J Path.2005; 206(3):291–304.

SemenzaGL. Angiogenesis in ischemic and neoplastic disorders. Annual Rev Med. 2003; 54:17–28.

CeradiniDJ, KulkarniAR,Callaghan MJ, et al. Progenitor cell traicking is regulated by hypoxic gradients through HIF-1 induction of SDF. Nat Med. 2004; 10(8):858–864.

Manalo D J, Rowan A, Lavoie T, etal. Transcriptional regulation of vascular endothelial cell responses to hypoxia by HIF-1.Blood. 2005;105(2):659–69.

Masoud G, Li W. HIF-1α pathway: role, regulation and intervention for cancer therapy. Acta Pharm Sin B.2015; 5(5):378-89.

Bastaki M, Nelli E, Dell'Era P, Rusnati M, et al. Basic fibroblast growth factor induced angiogen is phenotyre in mouse end othelium. A study of aortic and microvascular endothelial cell lines. Arterioscler Thromb Vasc Biol. 1997; 17: 454-64.

OrysykSI, ZholobOO, Bon VV, et al. Novel chelate complexes of Co(II), Ni(II), Cu(II), Pd(II) derived from anti- and syn-isomers of 2-(2-aminothiazole-4-yl)-2-hydroxyiminoacetic acid with pro-/antiproliferative actions on endothelial cells. Polyhedron. 2015;85:208–20.

NicolettiI,MiglioratiG,PagliacciMC,et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods.1991;139:271-80.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assayas. J Immunol Methods. 1983; 65:55-63.

Green LC, WagnerDA, GlogowskiJ, et al. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal. Biochem. 1982; 126(1):1318.

Nikolaienko Т, PetrukN, Shelest D, et al. Influence of VEGF, EGF and their antagonists on proliferative activity and glucose consumption by endothelial cells. Bull. of T. Shevchenko Nat. Univ. of Kyiv.2015; 69(1):36-8.



  • There are currently no refbacks.

Лицензия Creative Commons
This journal is available according to the Creative Commons License «Attribution» («Атрибуція») 4.0 Global (CC-BY).