Detergent-stable proteases from the antarctic scallop Adamussium colbecki

N. Raksha, D. Gladun
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv


Detergent-stable proteases were found in crude extract of the Antarctic scallop Adamussium colbecki. The results of gelatin zymography indicated the availability of the proteases which able to act in the presence of SDS, whereas plasmin and
miniplasmin maintained their activity toward gelatin only after complete removing of detergent. Total proteolytic activity, represented by casein hydrolysis was within the control level after incubation of crude extract with detergents such as Tween 80
and SDS whereas trypsin activity was significantly reduced.


marine hydrobiont Adamussium colbecki, SDS, Tween 80, ethanol, total proteolytic activity

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