Factors and their receptor genes in glioma cells with suppressed function of ERN 1 enzyme upon glucose deprivation

A. Kharkova, O. Minchenko
Taras Shevchenko National University of Kyiv, Kyiv; Palladin Institute of Biochemistry, Kyiv

Abstract


It was shown that the expression level of insulin like growth factors (IGF1 and IGF2) genes is decreased, but IGF receptor (IRF1R) gene is significantly increased in U87 glioma cells with suppressed activity of the sensor and signaling enzyme ERN1. In U87 glioma cells the expression level of IGF1 gene is decreased but IGF2 and IGF1R do not change significantly upon glucose deprivation condition. The inhibition of ERN1 functional activity does not affect the sensitivity of IGF1 and IGF1R gene expressions to glucose deprivation but the inhibition of ERN1 eliminates the effect of glucose deprivation on IGF2 gene expression. Thus, the IGF1, IGF2 and IGF1R genes are related to the regulation of glioma cells proliferation and are sensitive to glucose deprivation in dependence of ERN1 enzyme function.


Keywords


gene expressions, IGF1, IGF2, IGF1R, glioma cells, glucose deprivation, ERN1

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DOI: http://dx.doi.org/10.17721/2616_6410.2016.20.44-49

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