Stability of human recombinant AIMP1/Р43 protein in nanocomposite complex with beta-cyclodextrin

N. Vorobyova, O. Kornelyuk, D. Lozhko
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Institute of Molecular Biology and Genetics of NASU, Kyiv

Abstract


Stability of the recombinant AIMP1/p43 protein – component of aminoacyl-tRNA synthetase complex of higher eukaryotic – in nanocomposite complex with β-cyclodextrin was investigated. A significant increase in thermal stability AIMP1/p43 in the composition of nanocomposite complex was shown. The local conformational transition associated with the exposure of Trp271 residue on the AIMP1/p43 surface was observed at 43 0 C, but in the nanocomposite complex it was observed at 52 0 C. Stabilization of AIMP1/p43 protein in nanocomposite complex provides opportunities for further structural and functional studies and its use as a new biotechnology product in biomedicine.


Keywords


AIMP1/р43, β-cyclodextrin, stabilization, fluorescence spectroscopy

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DOI: http://dx.doi.org/10.17721/2616_6410.2016.20.15-18

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