Kisspeptin-mediated regulation of the morpho-functional state of the rat testicles under the effect of silver nanoparticles

V. Kalinovskyi, A. Pustovalov, M. Dzerzhynskyi, G. Grodzyuk, N. Andriushina
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Nanomedtech-LLC, Kyiv; Nanomedtech-LLC, Kyiv


It was shown that silver nanoparticles downregulate reproductive system of male rats. At the same time, activation of the hypothalamic sex-function regulatory center did not result in any stimulatory effect on testes. We suppose that toxicity of silver nanoparticles is due to their direct action on the testicles.


nanoparticles, silver, kisspeptin, testis

Full Text:



Matvienko MG, Pustovalov AS, Buzynska NO, Dzerzhinsky ME.Morphofunctional changes in 24-month rat testes under kisspeptin influence against blockade and activation of alpha-adrenergic receptors and melatonin administration. BMIR, 2012;95(2):170-3. Ukrainian.

Pryskoka AO. Acute intraperitoneal toxicity study of silver nanoparticles. Pharmacology and Drug Toxicology, 2014;37(1): 85-90. Ukrainian.

Lehman M, Merkley C, Coolen L, Goodman R. Anatomy of the kisspeptin neural network in mammals. Brain Res 2010;1364:90–102.

Lee J, Kim Y, Song K, Ryu H, Sung J, Park J, et al. Biopersistence of silver nanoparticles in tissues from Sprague–Dawley rats. Part Fibre Toxicol 2013;10:36.

Clarke H, Dhillo W, Jayasena C. Comprehensive Review on Kisspeptin and Its Role in Reproductive Disorders. Endocrinol Metab 2015;30:124–41.

Hong J-S, Kim S, Lee S, Jo E, Lee B, Yoon J, et al. Combined repeated-dose toxicity study of silver nanoparticles with the reproduction/developmental toxicity screening test. Nanotoxicology 2014;8:349–62.

Pineda R, Garcia-Galiano D, Roseweir A, Romero M, Sanchez-Garrido MA, Ruiz-Pino F, et al. Critical roles of kisspeptins in female puberty and preovulatory gonadotropin surges as revealed by a novel antagonist. Endocrinology 2010;151:722–30.

Asare N, Instanes C, Sandberg W, Refsnes M, Schwarze P, Kruszewski M, et al. Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology 2012;291:65–72.

Austin C, Umbreit T, Brown K, Barber D, Dair B, Francke-Carroll S, et al. Distribution of silver nanoparticles in pregnant mice and developing embryos. Nanotoxicology 2011;6:912–22.

Zande M, Vandebriel R, Doren E, Kramer E, Rivera Z, Serrano-Rojero C, et al. Distribution, elimination, and toxicity of silver nanoparticles and silver ions in rats after 28-day oral exposure. Acs Nano 2012;6:7427–42.

Ordzhonikidze CG, Ramaiyya LK, Egorova EM, Rubanovich AV. Genotoxic effects of silver nanoparticles on mice in vivo. Acta Naturae 2009;1:99–101.

Grachev P, Li X, Lin Y, Hu M, Elsamani L, Paterson S, et al. GPR54-Dependent Stimulation of Luteinizing Hormone Secretion by Neurokinin B in Prepubertal Rats. Plos One 2012;7.

Thakur M, Gupta H, Singh D, Mohanty I, Maheswari U, Vanage G, et al. Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration. J Nanobiotechnology 2014;12:42.

Lan Z, Yang W-X. Nanoparticles and spermatogenesis: how do nanoparticles affect spermatogenesis and penetrate the blood–testis barrier. Nanomedicine-Uk 2012;7:579–96.

Han J, Jeong J-K, Gurunathan S, Choi Y-J, Das J, Kwon D-N, et al. Male- and female-derived somatic and germ cell-specific toxicity of silver nanoparticles in mouse. Nanotoxicology 2015:1–13.

Matvienko MG, Pustovalov AS, Dzerzhinsky ME. Variety of functions and effects of kisspeptin. Biopolymers and Cell 2013;29:11–20.

Paxinos G., Watson C. The rat brain in stereotaxic coordinates. 6th ed. San Diego: Elsevier Academic Press;2007.

Creasy D, Bube A, de Rijk E, Kandori H. Proliferative and nonproliferative lesions of the rat and mouse male reproductive system. Toxicologic Pathology 2012;40:40S–121S.

Sanchez-Garrido M, Tena-Sempere M. Metabolic control of puberty: Roles of leptin and kisspeptins. Horm Behav 2013;64:187–94.

Lee Y, Kim P, Yoon J, Lee B, Choi K, Kil K-H, et al. Serum kinetics, distribution and excretion of silver in rabbits following 28 days after a single intravenous injection of silver nanoparticles. Nanotoxicology 2012;7:1120–30.

Gromadzka-Ostrowska J, Dziendzikowska K, Lankoff A, Dobrzyńska M, Instanes C, Brunborg G, et al. Silver nanoparticles effects on epididymal sperm in rats. Toxicol Lett 2012;214:251–8.

Skorupskaite K, George J, Anderson R. The kisspeptin-GnRH pathway in human reproductive health and disease. Human Reproduction Update 2014;20:485–500.

Melnik E, Buzulukov Y, Demin V, Demin V, Gmoshinski I, Tyshko N, et al. Transfer of Silver Nanoparticles through the Placenta and Breast Milk during in vivo Experiments on Rats. Acta Naturae 2013;5:107–15.



  • There are currently no refbacks.

Лицензия Creative Commons
This journal is available according to the Creative Commons License «Attribution» («Атрибуція») 4.0 Global (CC-BY).