Eeffect of an aquaeous extract of Phaseolus vulgaris pods on morphological properties of post-burn esophagus

Ya. Raetska
Taras Shevchenkа National University of Kyiv, Kyiv

Abstract


The most widespread chemical damage in children – burn of the esophagus. It occurs when accidentally swallowing substances that have in their composition alkali, acid or other caustic substances. The depth of burn depends on the concentration and time of contact of the mucous
membrane with the affecting agent. As a result of penetration of alkalis and contact with the wall of the esophagus, along with necrosis, damage to the mucous membrane, submucosal and muscular membrane of the esophagus occurs. Despite a large number of drugs that have been tested in the treatment of complications caused by esophageal burns, there is little information that would indicate the high effectiveness of the previously used drugs. The analysis of literature data shows the positive influence of polyphenols of natural origin on the normalization of physiological and biochemical parameters in various pathologies. The extract of common beans (Phaseolus vulgaris pods extract (PVPE)) is an example of a rich bioactive component of the source with beneficial characteristics for humans. Therefore, the purpose of our work was to investigate the effect of the Phaseolus vulgaris pods extract on the healing of post-burns wounds. When morpho-functional characteristics of the esophagus changes in the conditions of alkaline burn of the esophagus of the 2nd degree, it was shown that at 1 day of the experiment there is a violation of the mucous membrane, which manifests itself in the destruction of the multilayered epithelium, namely, in the submucous membrane there is a significant accumulation of leukocytes, leukocytic infiltration is fixed in the partly destroyed muscle layer. At 31 days of study in a group of animals with burn of the esophagus there is a certain restoration of the integrity of the mucous membrane of the esophagus, mainly with hyperplasia; there is also a
decrease in the presence of leukocytes; however, there is an enlargement of the connective tissue in the submucosal membrane, which may indicate the formation of a scar. The data of the morphological description are confirmed by the morphometric parameters of the calculation of the stenosis index. With the use of extracts of beans under chemical burns esophagus conditions, improvement of the morpho-functional status of the esophagus of rats was noted: the formed connective tissue scar does not significantly affect the stenosis index (its significance is the intermediate
position); the high level of leukocyte infiltration is reduced to the control parameters, which may indicate the obvious therapeutic effect of the
extract of beans pods at alkaline burns of the esophagus.

Keywords


alkali burn esophagus, gystology, cytokines, Phaseolus vulgaris

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References


Adam A. B., Özdamar M. Y., Esen H. H., & Günel, E. Local effects of epidermal growth factor on the wound healing in esophageal anastomosis: An experimental study. International Journal of Pediatric Otorhinolaryngology. 2017; 99, 8–12.

Bautista A, Varela R, Villanueva A, Estevez E, Tojo R, Cadranel S. Effects of prednisolone and dexamethasone in children with alkali burns of the oesophagus. Eur J Pediatr Surg 1996;6:198–203

Cakmak M, Nayci A, Renda N, Erekul S, Gökçora H, Yücesan S. The effect of corticosteroids and pentoxifylline in caustic esophageal burns. A prospective trial in rats. Int Surg 1997;82:371–5.

Cerit K, Karakoyun B, Bahadır E, Yüksel M, Bülbül N, Ercan F, Dağlı E, Yeğen B. The Effect of Antifibrotic Drug Halofuginone on Caustic Oesophageal Injury in Rats. Esophagus. 2018; 15:59–68.

Çiftçi Ö. D. The diagnostic utility of scintigraphy in esophageal burn: a rat model. Journal of surgical research. – 2016. (2): P. 495-500.

Cruz R, Pang TC, Harvey JG, Holland AJ. Chemical burns in children: Aetiology and prevention. Burns 2015; 41: 764-769

Contini S, Swarray-Deen A, Scarpignato C. Oesophageal corrosive injuries in children: a forgotten social and health challenge in developing countries. Bull. World Health Organ. 2009; 87:950-954.

Chibishev A, Simonovska N, Shikole A. Post-corrosive injuries of upper gastrointestinal tract. Prilozi. 2010; 31:297–316.

Contini S, Scarpignato C. Caustic injury of the upper gastrointestinal tract: a comprehensive review. World J Gastroenterol. 2013; 19:3918–3930.

Demirbilek S, Bernay F, Rizalar R, Bariş S, Gürses N. Effects of estradiol and progesterone on the synthesis of collagen in corrosive esophageal burns in rats. J Pediatr Surg 1994; 29:1425–8.

Dunbar KB, Agoston AT, Odze RD, Huo X, Pham TH, Cipher DJ, Castell DO, Genta RM, Souza RF, Spechler SJ. Association of Acute Gastroesophageal Reflux Disease With Esophageal Histologic Changes. JAMA. 2016; 315:2104–2112.

Fistal E.Y , G.P Kozinets, G.E Samoilenko and al., Combustiology, Kharkov: 2004.

Karaman İ., Orhan Koç, Ayşe Karaman Evaluation of 968 children with corrosive substance ingestion. Indian J Crit Care Med. 2015; 19(12): 714–718.

Koltuksuz U, Mutuş HM, Kutlu R, Ozyurt H, Cetin S, Karaman A, et al. Effects of caffeic acid phenethyl ester and epidermal growth factor on the development of caustic esophageal stricture in rats. J Pediatr Surg 2001; 36:1504–9.

Kyznetsova M.Y, Makieieva O.M, Lavrovska D.O, Tymoshenko M.O, Sheverova D.P, Halenova T.I, Savchuk O.M, Ostapchenko L.I. 2015. Effect of aqueous extract from Phaseolus vulgaris pods on lipid peroxidation and antioxidant enzymes activity in the liver and kidney of diabetic rats. J Appl Pharm Sci. 5:1–6.

Kraft R., Jensen K.-O., Jeschke M. G., & Schinkel, C. W. (2017). Patient With Scald Burn of the Esophagus. Journal of Burn Care & Research. 2017; 3: 468-470.

Öztorun C.İ., Demir S. The outcomes of becoming a pediatric burn center in Turkey. Ulus Travma Acil Cerrahi Derg. 2016; 22(1):34.

Oztan M. Arslan F., Oztan S., Diniz G., Koyluoglu G. Effects of topical application of platelet-rich plasma on esophageal stricture and oxidative stress after caustic burn in rats: Is autologous treatment possible? Journal of pediatric surgery. – 2018. (18) 30479-2

Raetska Y, Chornenka N, Koval T, Savchuk O, Beregova T, Ostapchenko L. Cytokine profile indicators in rat blood serum in a model of esophagus burn induced by antioxidant chemical preparation. Biomedical Research and Therapy [Internet]. 20 Sep. 2017.

Singer A.J, McClain S.A, Taira B.R, Guerriero J.L, Zong W. Apoptosis and necrosis in the ischemic zone adjacent to third degree burns. Acad Emerg Med. 2008;15:549–54.

Senturk, Ekrem et al. "Comparison of mitomycin-c and heparin affects in experimental corrosive esophagitis on rats." International journal of pediatric otorhinolaryngology. 2011; 785-9.

Tan J.Q, Zhang H.H, Lei ZJ, Ren P, Deng C, Li X.Y, et al. The roles of autophagy and apoptosis in burn wound progression in rats. Burns. 2013; 39:1551–6.

Tiwari V.K. Burn wound: how it differs from other wounds? Indian J Plast Surg. 2012; 45:364–73. 32.

Received: 01.03.2019

Revised: 02.04.2019

Signed for the press: 02.04.2019




DOI: http://dx.doi.org/10.17721/1728_2624.2019.26.6-12

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