Express control of levels of polyamines by immune biosensor based on SPR

M. Prylutskyi, N. Starodub, T. Lebyedyeva, P. Shpylovyy
National University "Kyiv-Mohyla Academy", Kyiv, Ukraine; National University of Life and Environmental Sciences of Ukraine, Kyiv; Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine, Kyiv; Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine, Kyiv

Abstract


Oncological diseases are very common throughout the population of many countries of the world, especially among middleaged
and elder people. The main threat of cancer diseases underlies in the difficulty of diagnosing them in the early stages. That
is why it is extremely important to search, create and work out the latest diagnostic methods for oncological diseases that would
allow to determine them more precisely and in shorter terms.The purpose of the work was to develop a method for qualitative
and quantitative analysis of spermine and spermidine polyamines as potential tumour markersusing an optical biosensor based
on the phenomenon of surface plasmon resonance. To conduct the research, methods of biosensor analysis were used. In the
course of the research, model solutions of spermine and spermidine polyamines were analyzed and changes in shifts of the resonance
angle of the biosensor depending on the concentration of the analytes were determined. According to the results obtained,
there is a reason to state that the developed biosensor technique allows detecting both spermine and spermidine in small
concentrations (detection range from 10 ng/ml to 100 ng/ml).Modification of the surface of the transducer with solutions of protein
A and bovine serum albumin not only improved the quality of the analysis, but also blocked the nonspecific binding sites of
the studied analytes with a sensitive layer of antibodies. In addition, it was shown that resonance angle shifts were larger for
spermine analysis than for spermidine, due to the differences in the spatial structure. According to the results, it has been concluded
that the use of surface plasmon resonance-based biosensor with surface modified by antibody to spermine or spermidine
is effective for quantitative and qualitative analysis of polyamines spermine and spermidine as potential tumour markers.


Keywords


surface plasmon resonance, spermine, spermidine, biosensor, tumour markers

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References


Bilynskyj B. T. Evolution of clinical problems in breast cancer and modern oncology progress. Oncology. 2010, 12 (3), 282–285 (In Ukrainian).

Mechev D. S., Moskalets O. I., Bondaruk O. S. Hormones and tumuor markers: clinical and methodological aspects (manual). Kyiv: "Medicine of Ukraine". 2007, 96 p. (In Russian).

Lodeiro C. Light and colour as analytical detection tools: a journey into the periodic table using polyamines to bio-inspired systems as chemosensors //Chemical Society Reviews. 2010, 39(8), 2948-2976..

Moskalets A. I., Shcherbina O. V.Tumour markers in laboratory diagnostics. Laboratory diagnostics. 2011, N 1, 64–72. (In Russian).

Tsutsui H. High-throughput LC–MS/MS based simultaneous determination of polyamines including N-acetylated forms in human saliva and the diagnostic approach to breast cancer patients //Analytical chemistry. 2013, 85 (24). 11835-11842.

Takayama T. Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry //ClinicaChimicaActa. 2016. 452, 18-26.

Levêque J. Polyamine profiles in tumor, normal tissue of the homologous breast, blood, and urine of breast cancer sufferers. Breast Cancer Research and Treatment. 2000, 60(2), 99–105.

Zaletok S. P., Berdinskikh N. К., Lialiushko N. М.Metabolism of polyamines in tumor tissues and in urine of patients with breast tumors. Oncology. 2000, 2 (4), 250–252. (In Ukrainian).

Zaletok S. P., Gogol S. V., Klenov O. O. Norarginine enhances the antitumor activity of inhibitors of polyamines synthesis in vivo. Oncology. 2017, 19 (1), 17–24. (In Ukrainian).

Tamkovych S. N., Vojcyckyj V. E. LaktyonovP. P.Modern methods of breast cancer diagnostics. Biomedical chemistry. 2014, 60 (2), 141–160. (In Russian).

Byun J. A. et al. Serum polyamines in pre-and post-operative patients with breast cancer corrected by menopausal status //Cancer letters. 2009, 273 (2) 300-304.

Miolo G. et al.Pharmacometabolomics study identifies circulating spermidine and tryptophan as potential biomarkers associated with the complete pathological response to trastuzumab-paclitaxel neoadjuvant therapy in HER-2 positive breast cancer //Oncotarget. 2016, 7 (26). 39809–39822

Kivirand K., Rinken T. Biosensors for biogenic amines: the present state of art mini-review //Analytical Letters. 2011, 44 (17), 2821-2833.

Dykman L. A., Hlebcov N. G.Golden nanoparticles in biology and medicine: achievements of recent years and prospects. ActaNaturae. 2011, 3 (2), 36–58. (In Russian).

Bonaiuto E. Ternary Hybrid γ‐Fe2O3/CrVI/Amine Oxidase Nanostructure for Electrochemical Sensing: Application for Polyamine Detection in Tumor Tissue //Chemistry–A European Journal. 2016, 22 (20), 6846-6852.

Starodub N.F. Antibody immobilization on the metal and silicon surface. The use of selfassembled layer and specific receptors. Bioelectrochemistry. 2005, 66 (1-2), 111–115.

Received in editorial: 2.10.2018

Received the revised version: 1.11.2018

Signed for press: 1.11.2018


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