Express control of levels of polyamines by immune biosensor based on SPR

M. Prylutskyi, N. Starodub, T. Lebyedyeva, P. Shpylovyy
National University "Kyiv-Mohyla Academy", Kyiv, Ukraine; National University of Life and Environmental Sciences of Ukraine, Kyiv; Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine, Kyiv; Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine, Kyiv


Oncological diseases are very common throughout the population of many countries of the world, especially among middleaged
and elder people. The main threat of cancer diseases underlies in the difficulty of diagnosing them in the early stages. That
is why it is extremely important to search, create and work out the latest diagnostic methods for oncological diseases that would
allow to determine them more precisely and in shorter terms.The purpose of the work was to develop a method for qualitative
and quantitative analysis of spermine and spermidine polyamines as potential tumour markersusing an optical biosensor based
on the phenomenon of surface plasmon resonance. To conduct the research, methods of biosensor analysis were used. In the
course of the research, model solutions of spermine and spermidine polyamines were analyzed and changes in shifts of the resonance
angle of the biosensor depending on the concentration of the analytes were determined. According to the results obtained,
there is a reason to state that the developed biosensor technique allows detecting both spermine and spermidine in small
concentrations (detection range from 10 ng/ml to 100 ng/ml).Modification of the surface of the transducer with solutions of protein
A and bovine serum albumin not only improved the quality of the analysis, but also blocked the nonspecific binding sites of
the studied analytes with a sensitive layer of antibodies. In addition, it was shown that resonance angle shifts were larger for
spermine analysis than for spermidine, due to the differences in the spatial structure. According to the results, it has been concluded
that the use of surface plasmon resonance-based biosensor with surface modified by antibody to spermine or spermidine
is effective for quantitative and qualitative analysis of polyamines spermine and spermidine as potential tumour markers.


surface plasmon resonance, spermine, spermidine, biosensor, tumour markers

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Received in editorial: 2.10.2018

Received the revised version: 1.11.2018

Signed for press: 1.11.2018


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