Effect of bloxopolymers on regulation and main indicators of the protein and carbohydrate metabolism in rats under conditions of subject toxicological experiment

O. Nakonechna, A. Bezrodnaya, K. Krivonos
Kharkiv National Medical University, Kharkiv; Kharkiv National Medical University, Kharkiv, Ukraine, Kharkiv National University named by V.N. Karazin, Kharkiv, Ukraine; Kharkiv National Medical University, Kharkiv, Ukraine, Kharkiv National University named by V.N. Karazin, Kharkiv, Ukraine

Abstract


In the process of a subacute toxicological experiment, the influence of a group of bloxopolymers (BP) in the composition of polyethylene glycol
(PEG-400), polypropylene glycol (PPG) and ethylene glycol (EG) on the main indicators of carbohydrate and protein metabolism and their regulation
in the rat organism was studied. According to the results of research, it was established that these xenobiotics (XB) in doses of 1/10 and 1/100 DL50
violate the regulation of carbohydrate and protein metabolism and their main indicators. Studies of the content of hormones that regulate protein
and carbohydrate metabolism in the blood of rats revealed a decrease in insulin, testosterone and estradiol. Analysis of the content of insulin
revealed its decrease in serum when exposed to PEG-400 at a doses of 1/10 and 1/100 DL50 by 54% and 38%, respectively. In terms of exposure to
PPG, the insulin content decreased by 51% and 30% depending on the dose. Toxification of EG rats contributed to a decrease in the insulin content
by 59% and 44 %%. The content of testosterone in male rats decreased by 52% and 41% under conditions of exposure to polyethylene glycol at a
doses of 1/10 and 1/100 DL50, respectively, and estradiol in females – by 47% and 42%. Toxification of rats with xenobiotic PPG in doses of 1/10 and
1/100 DL50 also led to a decrease in testosterone by 34% and 25%, and estradiol by 33% and 28%, respectively, for males and females. As a result
of evaluating the content of sex hormones under the conditions of exposure to the most toxic XB EG, they showed that the changes observed were
significant, namely: the testosterone content decreased by 71% and 57% under the conditions of exposure to EG at a doses of 1/10 and 1/100 DL50,
respectively, and estradiol – by 64% and 58%. Studies of monitoring indicators of carbohydrate and protein metabolism in the body of rats under
the influence of XB revealed a decrease in blood glucose and lactate. Evaluation of monitoring parameters of protein metabolism under the
influence of PEG-400, PPG and EG showed a decrease in total protein and urea against the background of increased creatinine.
According to the results of the subacute toxicological experiment, the presence of a violation of protein and carbohydrate metabolism and their
regulation in the rat body under the influence of the studied XB has been proven.


Keywords


xenobiotics, toxification, regulation, protein metabolism, carbohydrate metabolism

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Received in editorial: 2.10.2018

Received the revised version: 1.11.2018

Signed for press: 1.11.2018


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