Changes of morpho-functional indicators in blood of rats after the influence of feed additives of humic natural and for actions of stress factors

L. Diachenko, L. Stepchenko
Dnipro state agrarian and economic university, Dnipro; Dnipro state agrarian and economic university, Dnipro

Abstract


The regulation of vital functions and maintenance of the functional lability of the organism occurs due to the adaptation processes that are
implemented with the participation of the nervous, endocrine and immune systems. The results of the study of morpho-functional parameters
of blood of rats for the actions of water-immobilization combined stress (VICS) and after the influence of natural antioxidants of feed additives
of humic nature "Gumilid", "Eco-Impulse Animal" and vitamin E. The research was conducted on white sex- mature young male rats based on
vivarium. Animals were divided into 6 groups of 8 animals each. Group 1 (control) – intact animals. Rats 2,3,4,5, and 6 groups were modeled by
VICS. The following day, after the simulation of VICs of animals, 2 groups were withdrawn from the experiment, and 3,4,5 and 6 experimental
rats, water, feed additives and vitamin E were administered orally with a special dosage unit individually for 18 days, after which they were also
withdrawn from the experiment. Blood for research was taken from the heart (right ventricle) under thiopental anesthesia on the day the
animals were removed from the experiment. Under conditions of stress, there were significant changes in the morpho-functional parameters of
blood in rats, compared with the intact group of animals. A sharp decrease in the concentration of erythrocytes, hemoglobin and hematocrit
content indicates the possible development of the cellular response to the effects of VICs. The recovery period (18 days) after the action of
VICS caused the activation of adaptation processes, while the studied parameters remained significantly lower than those in intact animals. In
the blood of animals receiving the feed supplement "Gumilide" and vitamin E in the recovery period, they indicate an improvement in the
condition of the rats and the return of the studied parameters to the reference values. On the contrary, the use of the feed supplement "Eco
Impulse Animal" during this period did not cause marked changes in the morphological and functional parameters in the blood of rats
compared with animals that received purified water.


Keywords


erythrocytes, Gumilid, Eco Impulse Animal, stress

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Received in editorial: 2.10.2018

Received the revised version: 1.11.2018

Signed for press: 1.11.2018


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