Changes of morpho-functional indicators in blood of rats after the influence of feed additives of humic natural and for actions of stress factors

L. Diachenko, L. Stepchenko
Dnipro state agrarian and economic university, Dnipro; Dnipro state agrarian and economic university, Dnipro


The regulation of vital functions and maintenance of the functional lability of the organism occurs due to the adaptation processes that are
implemented with the participation of the nervous, endocrine and immune systems. The results of the study of morpho-functional parameters
of blood of rats for the actions of water-immobilization combined stress (VICS) and after the influence of natural antioxidants of feed additives
of humic nature "Gumilid", "Eco-Impulse Animal" and vitamin E. The research was conducted on white sex- mature young male rats based on
vivarium. Animals were divided into 6 groups of 8 animals each. Group 1 (control) – intact animals. Rats 2,3,4,5, and 6 groups were modeled by
VICS. The following day, after the simulation of VICs of animals, 2 groups were withdrawn from the experiment, and 3,4,5 and 6 experimental
rats, water, feed additives and vitamin E were administered orally with a special dosage unit individually for 18 days, after which they were also
withdrawn from the experiment. Blood for research was taken from the heart (right ventricle) under thiopental anesthesia on the day the
animals were removed from the experiment. Under conditions of stress, there were significant changes in the morpho-functional parameters of
blood in rats, compared with the intact group of animals. A sharp decrease in the concentration of erythrocytes, hemoglobin and hematocrit
content indicates the possible development of the cellular response to the effects of VICs. The recovery period (18 days) after the action of
VICS caused the activation of adaptation processes, while the studied parameters remained significantly lower than those in intact animals. In
the blood of animals receiving the feed supplement "Gumilide" and vitamin E in the recovery period, they indicate an improvement in the
condition of the rats and the return of the studied parameters to the reference values. On the contrary, the use of the feed supplement "Eco
Impulse Animal" during this period did not cause marked changes in the morphological and functional parameters in the blood of rats
compared with animals that received purified water.


erythrocytes, Gumilid, Eco Impulse Animal, stress

Full Text:



Belay IM, Tishkin VS. Investigation of hypolipidemic and antioxidant properties of picamilon and carnitine chloride. Ukrainian Rheumatological Journal. 2001;1 (3):21-25.

Buchko OM. Immunological and hematological parameters of blood of pigs for action of humic additive and ascorbic acid. Young Scientist.2005; 2 (17):25-28.

Griban VG, Efimov VG, RakyatinskyVM[et al.]. On the Effectiveness of the Use of Humene Preparations in Animal Husbandry and the mechanism of their effect on the organism. Scientific Bulletin of LNUVMBT named after SZ Gzhytsky. 2015; 17(3):63

Dubinina OY. Occasional stress and occidental modification of proteins. Medicine chemistry. 2001; 3 (2):5-12

Dyachenko LM, Stepchenko LM. The state of the erythrocytic system in the blood of rats against the background of the use of feed additives of humic nature under the influence of combined stress. Scientific and technical bulletin of NDC of biosafety and ecology. control of the resources of the agroindustrial complex. Forthcoming 2018

Efimov VG, RakyatinskyVM. Influence of humic substances on mineral exchange in cows. Scientific and technical bulletin of NDC of biosafety and ecology. control of the resources of the agroindustrial complex. 2012; 1(1):667. Efimov VG KostyshkevichKM, Rakytansky VM. Influence of supply of treated peat as additive on cellular immunity, antioxidant status and piglet productivity in early postpartum period. Human and Veterinary Medicine. 2016; 8 (3):133-136.

Mikhailenko EO, Domshina OO,StepchenkoLM. Protein and amino acid exchange in the muscles of broiler chickens COBB 500 cross with the use of the feed supplement "Gumilid". Scientific Bulletin of the Lviv National University of Veterinary Medicine and Biotechnology named after SZ Gzhytsky. 2017; 19(77)

Paronik VA,Stepchenko LM,DyachenkoLM, [et al.]. Influence of corvetane and humidide on the state of the oxidative-antioxidant system of rats on the background of adrenaline introduction. Biology of animals. 2015; 17(4):109-114.

Petrova GV,DonchenkoGV. The role of α-tocopherol in the oxidative stress of thymocytes in rat induced by hydrogen peroxide and menadion. Ukr. Biochemical Journal. 2008; 80(3)

Skorik. MV, Stepchenko LM. Interconnection of glutathione exchange of parameters of blood of hens-bearers against the background of a wormhomat. Veterinary medicine. 2006; 86:292-297.

Stepchenko LM,Skorik. MV. Condition of erythrocyte antioxidant laying hens for the actions of humic substances. Technical bulletin Scientific Institute of Animal Biology and the State Research Control Institute of Veterinary Preparations and Feed Additives. 2006: 7(3.4.):137-143.

Received in editorial: 2.10.2018

Received the revised version: 1.11.2018

Signed for press: 1.11.2018


  • There are currently no refbacks.

Лицензия Creative Commons
This journal is available according to the Creative Commons License «Attribution» («Атрибуція») 4.0 Global (CC-BY).