Content of TBA-active compounds and activity of antioxidant enzymes in rat serum under experimental osteoarthrosis and long-term administration of multiprobiotic

A. Vovk, O. Korotkyi, D. Yankonskyi, Ye. Torgalo, K. Dvorshchenko
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


The aim of the study was to investigate the effect of multiprobiotics on the content of lipid peroxidation products and antioxidant enzymes in
blood serum of rats during monoiodoacetate-induced osteoarthritis (MIA-induced OA).
The study was carried out on white non-linear, sexually mature male rats (weight 180-240g), according to general ethical principles of experiments
on animals. All animals were divided into four experimental groups. The first group – Control: animals got injection into knee ligament 0.05 ml of 0.9 %
NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14
days from the 8th to 22nd days. The second group – Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9 % NaCl solution on the first
day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking
water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved
in 0.05 ml of 0.9 % NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight
daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1
mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9 % NaCl on the first day of the experiment and then got intragastric administration 140 mg / kg
of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the
protocol of the ethics committee with rapid blood sampling. The content of TBA-active compounds was determined by reaction with thiobarbituric acid.
Superoxide dismutase activity was evaluated by the ability of the enzyme to compete with nitrosin tetrazolium for superoxide radicals. The catalase
activity was measured by the amount of undiluted hydrogen peroxide in the samples.
It has been established that MIA-induced OA disturbed oxidative-antioxidant balance of the rat serum: the content of TBC-active compounds
was increased, superoxide dismutase was reduced and catalase activity was increased. It was shown that with the long-term administration of
multiprobiotics in animals with MIA-induced OA, the above indicators were restored.


Keywords


monoiodoacetate-induced osteoarthritis, multiprobiotic, TBA-active compounds, superoxide dismutase, catalase, blood serum

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References


Cook C.S., Smith P.A. Clinical Update: Why PRP Should Be Your First Choice for Injection Therapy in Treating Osteoarthritis of the Knee // Curr. Rev. Musculoskelet. Med. – 2018. – Vol. 11(4). – P. 583-592.

Man G.S., Mologhianu G. Osteoarthritis pathogenesis – a complex process that involves the entire joint // J. Med. Life. – 2014. – Vol. 7, №1. – P. 37-41.

Bravo-Blas A., Wessel H., Milling S. Microbiota and arthritis: correlations or cause? // Curr Opin Rheumatol. – 2016. – Vol. 28(2). – P. 161-167.

X., Zeng B., Zhang J. et al. Role of the Gut Microbiome in Modulating Arthritis Progression in Mice // Sci. Rep. – 2016. – Vol. 6. – P. 30594.

Alipour B., Homayouni-Rad A., Vaghef-Mehrabany E. et al. Effects of Lactobacillus casei supplementation on disease activity and inflammatory cytokines in rheumatoid arthritis patients: a randomized double-blind clinical trial // Int. J. Rheum. Dis. – 2014. – Vol. 17(5). – P. 519-527.

Baragi V.M., Becher G., Bendele A.M., et al. A new class of potent matrix metalloproteinase 13 inhibitors for potential treatment of osteoarthritis: Evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models // Arthritis. Rheum. – 2009. – Vol. 60(7). – P. 2008-2018.

Стальная И.Д. Метод определения малонового диальдегида с помощью тиобарбитуровой кислоты / И.Д. Стальная, Т.Г. Гаришвили // Современные методы в биохимии. – М.: Медицина, 1977. – С. 66–68.

Чевари С. Роль супероксиддисмутазы в окислительных процессах клетки и метод определения ее в биологических материалах / С. Чевари, И. Чаба, Й. Секей // Лаб. дело. – 1985. – № 11. – С. 678–681.

Метод определения активности каталазы / М. Королюк [та ін.] // Лаб. дело. – 1988. – № 1. – С. 16–19.

Реброва О.Ю. Статистический анализ медицинских данных. – М.: Информполиграф, 2002. – 305 с.

Drevet S., Gavazzi G., Grange L., Dupuy C., Lardy B. Reactive oxygen species and NADPH oxidase 4 involvement in osteoarthritis // Exp Gerontol. – 2018. – Vol. 111. – P. 107-117.

Kwon H.Y., Choi S.Y., Won M.H., Kang T., Kang J.H. Oxidative modification and inactivation of Cu,Zn-superoxide dismutase by

,2'-azobis(2-amidinopropane) dihydrochloride // Biochim. Biophys. Acta. – 2000. – Vol. 1543(1). – P. 69-76.

Jung O., Marklund S.L., Xia N., Busse R., Brandes R.P. Inactivation of extracellular superoxide dismutase contributes to the

development of high-volume hypertension // Arterioscler. Thromb. Vasc. Biol. – 2007. – Vol. 27(3). – P. 470-477.

Demicheli V., Moreno D.M., Radi R. Human Mn-superoxide dismutase inactivation by peroxynitrite: a paradigm of metal-catalyzed tyrosine nitration in vitro and in vivo // Metallomics. – 2018. – Is. 5, №10. – P. 679-695.

Вплив окисного стресу на рівень експресії генів TGF–β і HGF у печінці щурів в умовах тривалої шлункової гіпохлоргідрії та за введення мультипробіотика Cимбітер / К.О. Дворщенко [та ін.] // Укр. біохім. журн. – 2013. – Т. 85, № 5. – С. 114–123.

Azad M.A.K., Sarker M., Wan D. Immunomodulatory effects of probiotics on cytokine profiles // Biomed. Res. Int. – 2018:8063647. doi: 10.1155/2018/8063647.

Probiotics prevent intestinal barrier dysfunction in acute pancreatitis in rats via induction of ileal mucosal glutathione biosynthesis / F. Lutgendorff [et al.] // PLoS ONE. – 2009. – Vol.4, № 2. – P. e4512.


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