The level of ADM, SLC1A3, HSPA5 and PDGFC gene expressions in obese adolescents and adult men

Y. Viletska, D. Minchenko, V. Davydov, O. Minchenko
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; SI "Institute of Children and Adolescent Health Care, NAMS Ukraine", Kharkiv; Palladin Institute of Biochemistry, Kyiv


The aim of this work was to analyze the relative level of the expression of ADM (adrenomedullin), SLC1A3 (glial high affinity glutamate
transporter), HSPA5 (heat shock protein family A member 5) and PDGFC (platelet derived growth factor C) genes, which encoding poly-functional
proteins, in adolescents blood and adipose tissue in adult men with obesity without insulin resistance to determine their possible role in the
development of obesity and its complications. It was shown that relative expression level of SLC1A3, HSPA5 and PDGFC genes in the blood of
obese adolescents without insulin resistance was significantly increased as compared to control group of relative healthy individuals of the same
age without signs of obesity. At the same time, the expression level of ADM gene did not change significantly in these obese adolescents. It was
also shown that in subcutaneous adipose tissue of obese adult men without insulin resistance the relative level of SLC1A3 and PDGFC gene
expressions was decreased, but ADM and HSPA5 genes – increased as compared to control group. The increased level of ADM gene expression,
which has hypotensive activity, controls corticotrophin, leptin, endothelin-1 and adiponectin secretion and is related to the development of insulin
resistance and metabolic syndrome as well as overexpressed in malignant tumors, possibly related to the development of obesity complications
including tumorigenesis. To a large extent, this also applies to increased expression of HSPA5 gene, which is involved in controlling various
metabolic pathways, both in and outside cells, found in the endoplasmic reticulum, nucleus, mitochondria, and cytosol, plays an important role in
the endoplasmic reticulum stress, obesity, insulin resistance and carcinogenesis. Therefore, the expression level of genes, which related to the
development of obesity and endoplasmic reticulum stress as well as proliferation processes, was significantly changed in the blood and adipose
tissue at obesity in gene-specific manner.


gene expressions, ADM, SLC1A3, HSPA5, PDGFC, blood, adipose tissue, obesity

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Received in editorial: 26.09.2018

Received the revised version: 29.10.2018

Signed for press: 29.10.2018


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