The combined influence of the intermittent normobaric hypoxia and melatonin on morphofunctional activity of the rat's liver parenchyma

R. Yanko
O. O. Bogomoletz Institute of Physiology National Academy of Science of Ukraine, Kyiv

Abstract


The aim of the study was to investigate the morphological changes in the liver parenchyma of rats after the combined effect of intermittent
normobaric hypoxia (INH) and melatonin.
The study was carried out in the spring on 24 male rats of the Wistar line. The experimental animals were daily given a hypoxic gas
mixture (12 % oxygen in nitrogen) in the intermittent mode: 15 minutes deoxygenation / 15 minutes reoxygenation for 2 hours. The same
rats were daily administered orally with exogenous melatonin at 10.00 at a dose of 5 mg / kg. The duration of the experiment was 28 days.
Histological preparations from the liver tissue were prepared according to a standard procedure. Morphometry was performed using the
computer program "Image J". In rats exposed to INH and melatonin, a significantly larger area of hepatocytes, their nucleus and cytoplasm
were detected by 19, 54 and 31 %, respectively, than in the control. This led to a significant increase in the nuclear-cytoplasmic ratio by
35 %. The experimental animals showed a higher number of nucleolus in the nucleus of hepatocytes (by 14 %) and the number of binuclear
hepatocytes (by 23 %). These data may indicate an increase in the functional activity of hepatocytes, the activation of physiological cell
regeneration at the intracellular level. Also, experimental animals showed a decrease in the distance between the nucleus of adjacent
hepatocytes, which indicates a more dense arrangement of cells among themselves and a decrease in the amount of intercellular
connective tissue. The combined effect of intermittent normobaric hypoxia and melatonin has morphological signs of increased functional
activity and physiological regeneration of the liver parenchyma.


Keywords


liver, intermittent hypoxia, melatonin

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References


Berezovsky VA. Natural and instrumental orotherapy. Donetsk: "Zaslavsky A.Yu.", 2012. (Russian).

Powell F, Garcia N. Physiological effects of intermittent hypoxia. High. lt. Med. Biol. 2002; 1:125–36.

Serikov VS, Lyashev YuD. Effect of melatonin on stress-induced changes in liver of rats with different resistance to stress. Bjulleten' jeksperimental'noj biologii i mediciny. 2015; 3: 290–94. (Russian).

Chojnacki C, Walecka-Kapica Е, Romanowski М, Chojnacki J, Klupinska G. Protective role of melatonin in liver damage. Curr Pharm Des. 2014; 20(30): 4828–33.

Pevet P. Melatonin and biological rhythms. Therapie. 1998; 30(1):109–124.

Zavodnik LB, Zavodnik IB, Lapshina EA, Belonovskaya EB, Martinchik DI, Kravchuk RI, Bryszewska M, Reiter RJ. Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats. Cell Biochem.

Funct. 2005; 23:353–59.

Abbasoglu O, Berker М, Ayhan А, Palaoglu S, Sayek I. The effect of the pineal gland on liver regeneration in rats. J. Hepatol. 1995; 23(5):578–81.

Arkhipenko Y, Sazontova Т, Zhukova А. Periodic hypoxia and hyperoxia improves resistance of membrane structures in heart, liver and brain. Bulletin of Experimental Biology and Medicine. 2005; 140(3):278–81.

Solís-Muñoz P, Solís-Herruzo JA, Fernández-Moreira D, Gómez-Izquierdo E, García-Consuegra I, Muñoz-Yagüe T, García Ruiz I. Melatonin improves mitochondrial respiratory chain activity and liver morphology in

ob/ob mice. J. Pineal Res. 2011; 51(1):113–23. doi: 10.1111/j.1600-079X.2011.00868.x.

Zhuravleva SA. Histology. Workshop. – Minsk: "The High School", 2013. (Russian).

Lebedevа EI. Morphometric parameters of hepatocytes of white rats and humans in toxic liver cirrhosis. Universum: Medicina i farmakologija: electron. scientific. journal. 2015; 7-8: URL: http://7universum.com/en/med/archive/item/2547. (Russian).

Sarkisov DS. Electron microscopy of destructive and regenerative intracellular processes. – Moscow: "Medicine", 1967. (Russian).

Boisvert F, Konningsbruggen S, Navascues J, Lamond AI. The multifunctional nucleolus. Nature Reviews. Molecular Cell Biology. 2007; 8(7):574–85. doi:10.1038/nrm2184.

Romanova LP, Malyshev AI. The role of binuclear hepatocytes in liver regeneration after mechanical trauma in early ontogenesis in rats. Vestnik Chuvashskogo universiteta. 2011; 3: 398-402. (Russian).

Yanko RV, Chaka OG, Plotnikova LM. Comparison of the state of liver parenchyma of rats cut off lines after the administration of melatonin. Ukraїns'kij zhurnal medicini, bіologії ta sportu. 2015; 1(1): 201-6. (Ukraine).

Yanko RV, Berezovsky VA, Levashov MI. Influence of intermittent hypoxia on the morphofunctional state of the thyroid and liver. Rossijskij fiziologicheskij zhurnal imeni І.М. Sechenova. 2017; 103 (5):553-61. (Russian).

Lebkova NP, Chizhov AY, Bobkov Yu.I. Adaptive intracellular mechanisms of regulation of energy homeostasis with intermittent normobaric hypoxia. Rossijskij fiziologicheskij zhurnal imeni Sechenova.

; 85 (3): 403-11. (Russian).

Michael SW, Jan SJ, Christian R, Charlotte KS, Jan HH. Widespread hypoxia-inducible expression of HIF-2a in distinct cell populations of different organs. Faseb J. 2003; 17:271–73.

Liang R, Nikholgh A, Hoffmann K, Kern M, Schneider H, Sobirey M, Zorn M, Büchler MW, Schemmer P. Melatonin protects from hepatic reperfusion injury through inhibition of IKK and JNK pathways and modification of cell proliferation. J. Pineal Res. 2009; 46(1):8–14.

Cini G, Neri B, Pacini A, Cesati V, Sassoli C, Quattrone S, D'Apolito M, Fazio A, Scapagnini G, Provenzani A, Quattrone A. Antiproliferative activity of melatonin by transcriptional inhibition of cyclin D1 expression: a molecular basis for melatonin-induced oncostatic effects. J. Pineal Res. 2005; 39:12–20.

Received in editorial: 26.09.2018

Received the revised version: 29.10.2018

Signed for press: 29.10.2018


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