Free-radical processes in serum of rats under conditions of carrageenan-induced inflammation of the hind limb and prolonged prophylactic administration of chondroitin sulfate

O. Blokhina, A. Dranitsina, L. Kot, V. Khilko, K. Dvorshchenko
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv

Abstract


The aim of the work was to investigate the preventive effect of Chondroitin Sulfate on the content of reactive oxygen species and lipid
peroxidation products in blood serum of rats at local inflammation of the hind limb.
The studies were conducted on white non-linear, sexually mature male rats weighing 180–240 g, in compliance with the general ethical
principles of experiments on animals. All animals were divided into four experimental groups. The first group – control: animals sub-planar injected
0.1 ml of 0.9 % NaCl solution into the posterior right limb. The second group – animals received a therapeutic dose of 3 mg x kg-1 chondroitin
sulfate daily for 28 days daily. The third group – animals were infused intramuscularly with 0.1 ml of 0.9 % NaCl solution in the posterior right limb
for 28 days and for 29 days inflammatory edema of the limb was stimulated (animals were sub-planar injected with 0.1 ml of 1 % carrageenan
solution to the posterior right limb ) The fourth group – for 28 days rats were daily intramuscularly injected with a therapeutic dose of 3 mg x kg-1
chondroitin sulfate, after which on 29th day, inflammatory edema of the limb was stimulated. The total number of animals involved in experimental
studies was 40 individuals. The content of the superoxide radical was determined by the level of XTT-formazan. The level of hydrogen peroxide was
measured in the sorbitol-xylenol orange system. The content of diene conjugates was determined in the heptane-isopropanol extract by the
spectrophotometric method. The content of Schiff bases was determined by the fluorimetric method. The content of TBA-active compounds was
determined by the reaction with thiobarbituric acid.
It has been established that with carrageenan-induced inflammation of the posterior limb, the content of reactive oxygen species and lipid
peroxidation products increases in the serum. It was shown that the prophylactic administration of chondroitin sulfate based drug on animals with
carrageenan-induced inflammation restored the abovementioned parameters.


Keywords


acute inflammation of the limb, chondroitin sulfate, reactive oxygen species, lipid peroxidation, blood serum

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Received in the editorial: 10.09.2018

Received a revised version: 8.10.2018

Signed for the press: 8.10.2018


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