Adhesive, migration and lactate dehydrogenase activity in breast cancer cells under activation TLR–ligand teichoic acid

A. Nehelia, V. Nikulina, N. Senchylo, Ie. Torgalo, L. Garmanchuk
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv

Abstract


Toll-like receptors are cell receptors, the ligands of which are mostly antigens of bacteria and viruses. Their activation triggers multiple cascades that cause the body's immune response. The atypical location of toll-like receptors is often observed in various types of tumor diseases. In view of this, various approaches to the combination of antitumor therapy with the activation of the toll-like receptors are considered. In particular, one of the most common is toll-like receptor type 4, which activator is teichoic acid. In a previous study, it was shown that teichoic acid and PO244 combined therapy increases the apoptotic level of LLC cells and reduces the number of cells in a proliferative pool of cell cycle.One of the most important indicators of tumor diseases is the ability of tumor cells to migrate, which characterizes the extent of their degeneration and
affects the ability to vascularisation and invasion. This ability is directly dependent on the adhesive properties of tumor cells. Another feature of tumor cells is the change in biochemical properties, in particular, increased glucose consumption and excessive activation of glycolysis, followed by its fermentation to lactate.In the conditions of 3D and 2D growth, the effect of TLR ligand teichoic acid on migration, adhesive and lactate dehydrogenase activity of MCF-7 mammary gland cancer cells was investigated. A nearly two-fold increase in the population of MCF-7 attached cells was detected under the influence of teichoic acid. Also more than double increase in the rate of attachment of cells in unfed culture conditions was demonstrated, indicating an increase in the adhesion characteristics of MCF-7 tumor cells under the action of teichoic acid. Under the influence of teichoic acid the tumor cell migration was reduced 1.5 times, which may be a sign of a metastatic decreasing and inactivation of lactate dehydrogenase, indicating biochemical changes in the whole cell.


Keywords


teichoic acid, MCF-7 cells, adhesive properties, LDH-activity

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Received: 12.03.2018

Revised: 16.04.2018

Signed for publication: 16.04.2018




DOI: http://dx.doi.org/10.17721/2616_6410.2018.24.31-36

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