Influence of vanadium citrate on lactatdehydrogenase activity in bloody erythrocytes and tissues of rats with experimental diabetes

H. Klymets, R. Iskra
Institute of Animal Biology NAAS, Lviv; Institute of Animal Biology NAAS, Lviv

Abstract


The influence of vanadium citrate on the activity of lactate dehydrogenase in erythrocytes of blood and liver, kidney and skeletal muscle tissues of the experimental rats with induced diabetes was studied. Animals were divided into five groups: I – control, II, III, IV and V – experimental. Rats of the 1st and 2nd groups were given pure water without additives to drink, and animals of the groups ІІІ, ІV and V were given drinking water, to which the solution of vanadium citrate in the amount of 0,125, 0,5 and 2,0 μg V/ml of water was added. The determination of lactate dehydrogenase activity in erythrocytes and tissues was carried out by spectrophotometric method, based on the use of conjugated oxidation systems of nicotinamide coenzymes. Under experimental diabetes, a significant increase in lactate dehydrogenase activity was observed in blood erythrocytes, skeletal muscles and kidneys. However, in the liver, a significant decrease in the enzyme activity was observed according to the control. The increase in lactate dehydrogenase activity in erythrocytes under diabetes is due to the intensification of glycolysis processes. The increase in this enzyme activity in muscles causes lactic acidosis. High levels of glucose and hypertension play a major role in the development of diabetic nephropathies and increase in lactate dehydrogenase activity in kidneys. The decrease in the enzyme activity in the liver under diabetes is due to the occurrence of oxidative stress, which leads to its damage. Watering vanadium citrate to experimentally diabetic rats promotes normalization of the activity of carbohydrate metabolism enzymes, particularly glycolysis. Vanadium citrate normalizes glucose level in the blood, favorably affects erythropoiesis, accelerates the maturation of red blood cells, enhances antioxidant defense in the cells of body tissues, which promotes normalization of lactate dehydrogenase activity in tissues and erythrocytes with the approximation of its activity indicators to control values.


Keywords


vanadium citrate, lactate dehydrogenase, erythrocytes, tissues, diabetes, rats

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Received: 05.02.2018

Revised: 12.03.2018

Signed for publication: 12.03.2018




DOI: http://dx.doi.org/10.17721/2616_6410.2018.24.24-27

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