N. Chornenka, K. Valetska, Ya. Rayetska, L. Ostapchenko
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine


During the esophageal burns first and second degree, were observed the increase of total proteolytic activity and of the main proteinase (metalloproteinases and serine). Experimental melanin correction at a dose of 0.1 mg / kg contributed to the normalization of the pathological increase of total proteolytic activity and the activity of the main proteinase (metalloproteinases and serine), which indicates the normalization of the proteolysis system. 


alkaline burn of the esophagus; proteolysis; melanin

Full Text:



Ishchuk TV., Savchuk O.M, Raetska YaB, Vereschaka VV., Ostapchenko LI. Functioning proteases in the esophagus mucosa after chemical burns. Physiological Journal. 2015; 61. 5

Zvyagintsev TV, AV Krivoshapka, EV Zhelnin. Effect of wound healing drugs with different mechanisms of action to ukroven proinflammatory cytokines in burn injury in rats. Experimental and Clinical Medicine. 2011. №1 (50) P.57-60

Raetska Y, Ischuk T, Dzhus O, Savchuk O, Ostapchenko L. Experimental modeling of 1st and 2nd degrees alkali esophageal burn in immature rats. Biological system. 2014; 6(1): 39 -44.

Ishchuk TV., Koval TV., Savchuk OM., Raetska YaB., Ostapchenko LI. Dynamics Changes of Proteolytic Balance in Blood Plasma under Experimental Chemical Burns of Esophageal Development in Rats International Journal of Health Sciences & Research. 2016. 259. 12.

Abaev JK Biology healing acute and chronic wounds. Med. News, 2003;


Wolf K. Friedl P. Extracellular matrix determinants of proteolytic and non-proteolytic cell migration. Trends Cell Biol. 2011; 12. P. 736–744.

Beynon R, Judith S.Bond Proteolytyc enzymes : a practical approach. Oxford : Oxford University Press. 2001; 340 p.

Bořivoj K. Specificity of proteolysis / Bořivoj. Berlin: Springer-Verlag, 1992; 336 p

Hiemstra PS. Novel roles of protease inhibitors in infection and inflammation. Biochem Soc. Trans. 2002; №2. 116 – 120.

Kirpichenok LN The value of the components of proteolysis system in the regulation of inflammatory responses Medico-biological problems of life. 2010; № 2.15- 23.

Klimenko MO, Netyuhaylo LG. Burn disease (pathogenesis and treatment). 2009; 118.

Hetyuhaylo L.G Mechanisms of burn disease and justification of the drug "Kriohor" for its treatment: Author. Dis. on competition sciences. Dr. med degree. Sciences specials. 14.03.04 "Pathologic Physiology", 2007. 34. Kharkiv

Fistal E.Y, Kozinets G.P, Samoilenko G.E and al. Combustiology. 2004; 184. Kharkiv.

Seniuk O F., Gorovyi L.F., Palamar L.A., Krul N I.Effects of melaninglucan complex, isolated from polypore fungi, on the lifespan of female icr mice Probl. aging and longevity; 2014; 23, № 1. 11–27

Kazi A., Daniel K.G., Smith D.M., Kumar N.B., Dou Q.P. Inhibition of the proteasome activity, a novel mechanism associated with the tumor cell apoptosis-inducing ability of genistein. Biochem. Pharmacol; 2003. 66. 965–976.

Bahramsoltani R., Farzaei M.H., Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: An integrative review. Arch. Dermatol. 2014. 306. 601–617.

Yoo HG., Lee B.H., Kim W., Lee J.S., Kim G.H., Chun O.K., Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress. J. Med. Food; 2014;17. 1189–1196.

Samoylenko A., Hossain J.A., Mennerich D., Kellokumpu S., Hiltunen J.K., Kietzmann T. Nutritional countermeasures targeting reactive oxygen species in cancer: From mechanisms to biomarkers and clinical evidence. Antioxid. Redox Signal; 2013;

2157–2196. 19. Liaudanskas M., Viskelis P., Raudonis R., Kviklys D., Uselis N., Janulis V. Phenolic composition and antioxidant activity of Malus domestica leaves. Sci. World J; 2014; 306217.

Alov P., Tsakovska I., Pajeva I. Computational studies of free radical-scavenging properties of phenolic compounds. Curr. Top. Med. Chem; 2015; 15. 85–104.

Andjelkoviж M., Camp J. van, Meulenaer, Depaemelaere G., Socaciu C., Verloo M., Verhe R. Iron-chelation properties of phenolic acids bearing catechol and galloyl groups. Food Chem; 2006; 98. 23–31.

Chyzhanska NV, Tsyryuk OI, Beregova TV. The level of cortisol in the blood of rats before and after stress action against the background of melanin. Visnik of problems of biology and medicine; 2007; 1. 40-44.

Brenner M. Hearling VG. The protective role of melanin against UV damage in human skin. Photochem Photobio; 2008; Vol. 84(3). 539-549.

Romanovskay VA, Tashirev AB, Shilin SO, Chornaia NA. Resistance to UV radiation of microorganisms isolated from Antarctic rock habitats. Mіkrobіologіchny Journal; 2010;72, №3. 8–14.

Keypour S., Riahi H., Moradali M., Rafati H.. Investigation of the antibacterial activity of a chloroform extract of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae), from Iran. Int. J. Med. Mushrooms; 2008; 10, № 4. 345–349.

Racca S., Spaccamiglio A., Esculapio P., Abbadessa G., Cangemi L., DiCarlo F., et al. Effects of swim stress and alpha-MSH acute pre-treatment on brain 5-HT transporter and corticosterone receptor. Pharmacol Biochem Behav; 2005; 81(4). 894-900.

Chornenka NM., Raetska YA.B, Savchuk O.M. Torgalo E.O., Beregova T.V., Ostapchenko L.I.. Correction Parameters of Endogenous Intoxication in Experimental Burn Disease at the Stage of Toxemia. RJPBCS 7(5), 2016; 1042

Seniuk O., Gorovoj L., Kovalev V.. et al.Anticancerogenic propertis of melaninglucan complex from higher fungi. Proc. 5th Internat. Med. Mushroom Con. 2009; 142–149.

Golyshkin D.V., Falalyeyeva T.M., Chyzhanska N.V., Beregova T.V, Ostapchenko L.I. White blood count of rats under stress-induced stomach lesions and the prophylactic administration of melanin. Ukrainian Antarctic Journal; 2015; № 14. 114

Sukhomlyn A.A. Experimental correction of proteinase inhibitor imbalance by melanin in salivary glands under hypergastrinemia conditions The journal Ukrainian Medical Dental Academy; 2013; 23

Raetska Ya.B., Ishchuk T.V., Savchuk O.M., Ostapchenko L.I.. Experimental modeling of first-degree chemically-induced esophageal burns in rats. Medical chemistry; 2013; 15, № 4. 30-34.

Fistal EY, Kozinets G.P, Samoilenko G.E and al. Combustiology. Kharkov; 2004; 184 p.

Hummel ВС. Canadian Journal of Biochemistry and Physiology. 1956; №37. 1393–1995.

Wolf KP. Friedl. Extracellular matrix determinants of proteolytic and non-proteolytic cell migration. Trends Cell Biol. 2011; 21, №12. 736–744.

Hibbetts KB. Hines, D. Williams. An Overview of Proteinase Inhibitors. J Vet Intern Med. 1999;. 13. 302–308.

Veremeyenko KN, Goloborodko OP, Kizim AI Proteolysis in normal and pathological. Kiev: Health Protection 1988; 199

Luonut AG., Dindelegan, C. Ciuce. Proteases and Delayed Wound Healing Adv Wound Care. 2013; №8. 438–447.

Barrett AF. Woessner, N. Rawling. Handbook of Proteolytic Enzymes. (Academic Press), London, 2012; 4104

Petrenko O., Tikhomirov OA. The Role of matrix metalloproteinases in the physiological processes of wound healing. Medical and Clinical Chemistry. 2013; №4. 100-106.


  • There are currently no refbacks.