THE INDICATORS OF THE IMMUNE SYSTEM AND ANTIOXIDANT IN THE BLOOD OF PREGNANT FEMALE RATS F1 BY ACTION OF DIFFERENT DOSES OF GERMANIUM CITRATE

M. Khrabko, R. Fedoruk, S. Kropuvka
; Institute of animal biology NAAS, Lviv, Ukraine; Institute of animal biology NAAS, Lviv, Ukraine

Abstract


The aim of research was to study the effect of different doses of Ge citrate, obtained by nanotechnology method and chemically synthesized, the activity of their immune and antioxidant system blood of pregnant female rats. Research performed on rats F1 which were watering Ge citrate obtained by nanotechnology method in doses 10 (II), 20 (III), 200 (IV) mcg Ge and chemically synthesized Ge citrate in dose 2,000 (V) mg Ge. Ge citrate watering during the milk-feeding period up to 18-20 days of pregnancy. Not the same directional effect Ge citrate parameters of immune and antioxidant systems with increased content of immune globulin, glycoproteins and activity of enzymes of antioxidant protection that is more pronounced in females receiving 20 mcg Ge. Established inhibitory effect of Ge citrate 2 mg/kg albumin content and threeacylglycerol, amid increasing the level of creatinine in the blood of pregnant females V group which may indicate a decrease of albumin-transport function of filtering and absorption the ability of the kidneys.

Keywords


germanium citrate; blood; rat;, immunophysiology

References


Vlizlo VV, Iskra RJ, Fedoruk RS. Nanobiotechnology. Present state and future prospectes. The Animal Biology. 2015, 17 (4):18-29. [Ukrainian]

Dolaychuk OP, Fedoruk RS, Kaplunenk VG. Physiological effects nanotsytratu Ge in the conditions of his watering lactate female rats and their offspring / Physiological Journal. 2014. 60 (3): 222-223. [Ukrainian]

Stadnik AM, Byts GA, Stadnyk OA. The biological role of germanium in animals and humans. Scientific Herald of LNAVM Gzhytsky SZ. 2006, 8 (2):174-185. [Ukrainian]

Borisevich VB, Kaplunenko VG, Kosinov NV, at al. Nanomaterials in biology. Fundamentals nanoveterinary. K.: WA "Avicenna", 2010: 416 p. [Ukrainian]

Menchikov LG, Ignatenko MA. Biological activity of organogermanium compounds (a review). Pharmaceutical Chemistry Journal, 2012, 46 (11): 3-6. [Ukrainian]

Seifullina II, Martsinko EE, Afanasenko EV. Design and synthesis of new homo- and heterometal coordination compounds of germanium (IV) for preparation of low toxic drugs with a wide therapeutic action. Bulletin of the Odessa National University. Chemistry, 2015, 20 (4): 6-17. [Ukrainian]

Goodman S. Organic Germanium – Powerful Healer. J. Comp. Med., 1987, 4: 34-52.

Long QC, Zeng GX, Zhao XL. Pharmacocinetics of germanium after po beta-carboxyethilgermamum sesquioxide in 24 Chinese volunteers. Zhongguo Yao Li Xue Bao, 1996, 17 (5): 415-418.

Kresyun VI, Shemonayeva KF, Vidavska AG. Pharmacological characterization of compounds of germanium. Clinical Pharmacy, 2004, 4: 65-68. [Ukrainian]

Fedoruk RS, Khrabko MI, Tsap MM, Martsynko OE. Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium. Animal biology, 2016, 18 (3): 97–106. [Ukrainian]

Khrabko МІ, Fedoruk RS. Growth and development of F1 male rats organism and its immunophysiological activity during the period watering them different doses of nanotechnology and chemically synthesized germanium citrate. Bulletin of Kyiv National Taras Shevchenko University. Problems of regulation of physiological functions, 2016, 2 (21): 39-43. [Ukrainian]

Kosinov MV, Kaplunenko VG, 2009. Method metal carboxylates "Nanotechnology receiving metal carboxylates". Ukraine. Pat. 38391. [Ukrainian].

European convention for the protection of vertebrate animals used for experim. and other scientific purposes. Coun. of Europe, Strasbourg, 1986: 53.

Law of Ukraine № 3447-IV "On protection of animals from cruelty". Supreme Council of Ukraine. Official. kind. 2006, 27, 990: 230. (Library official publications). Kovalenko L. Evaluation nanoakvahelat germanium stimulating action on the natural resistance of animals. Scientific Herald NUBiP Ukraine, 2012, 172 (1): 203–209.

Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of investigation in biology, stock-breeding and veterinary. Reference book. Lviv. Spolom. 2012: 764. [Ukrainian]


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