CONDITION OF GLUTATHIONE SYSTEM IN THE SERUM OF RATS IN EXPERIMENTAL OSTEOARTHRITIS AND ADMINISTRATION OF CHONDROITIN SULPHATE

E. Tikhova, M. Tymoshenko, Y. Kovelska, K. Dvorshchenko
; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

Abstract


It was established that in osteoarthritis induced by administering of sodium monoiodacetate in the blood serum of rats glutathione system enzyme activities reduced – glutathione peroxidase, glutathione, oxidized glutathione and the ratio of redox glutathione forms moved towards oxidation. It has been found that after the introduction of the drug based on the sodium chondroitin sulphate enzyme activities grew and glutathione balance normalized in the serum of rats with chemically induced osteoarthritis.

Keywords


osteoarthritis; oxidation; glutathione; chondroitin sulfate

References


Dejour H. et al. Arthrosis of the knee in chronic anterior laxity. Orthopaedics & Traumatology: Surgery & Research 2014;100(1):49-58.

Philp A. M. et al. eNAMPT is localised to areas of cartilage damage in patients with hip osteoarthritis and drives cartilage catabolism leading to

proteoglycan loss and inflammation. Osteoarthritis and Cartilage 2016;(24):388.

Иманаева А. Я., Залялютдинова Л. Н. Патогенетическое обоснование применения антиоксидантов в лечении ревматоидного артрита. Современные проблемы науки и образования 2013;(2).

Yui N. et al. Mechanical and oxidative stress in osteoarthritis. The Journal of Physical Fitness and Sports Medicine 2016;5(1):81-86.

Meister A., Anderson M.E. Glutathione. Ann. Rev. Biochem. 1983;52:711–760.

Кулинский В.И., Колесниченко Л.С. Система глутатиона 1. Синтез, транспорт глутатионтрансферазы, глутатионпероксидазы. Биомед. Химия 2009;55(3):255–277.

Гуськова ТА. Доклиническое токсикологическое изучение лекарственных средств как гарантия безопасности проведения их клинических исследований. Токсикологический вестник 2010;5:2-5.

Hartree EF. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical biochemistry 1972;48(2):422-427.

Власова С.Н. Активность глутатионзависимых ферментов эритроцитов при хронических заболеваниях печени у детей. Лаб. Дело 1990;(8):19–22.

Hissin P.J. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical Biochemistry 1976;74(1):214–226.

Mokrasch L.C. Glutathione content of cultured cells and rodent brain regions: a specific fluorometric assay. Analytical Biochemistry 1984;140(2):506–509.

Наследов АД. Математические методы психологического исследования. СПб.: Речь 2006;166.

Tappel A. Selenium-glutathione peroxidase: properties and synthesis. Current topics in cellular regulation 2014;24:87-96.

Александрова Л. А. и др. Оценка проявлений окислительнонитрозильного стресса при системных заболеваниях соединительной ткани. Ученые записки СПбГМУ им. акад. ИП Павлова 2016;23(1):26-29.

Paglia D. E., Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Translational Research 1967;70(1):158-169.

S.S. Singhal, S.P. Singh, P. Singhal, D. Horne, J. Singhal, S. Awasthi, Antioxidant role of glutathione S-transferases: 4-Hydroxynonenal, a key molecule in stress-mediated signaling, Toxicol. Appl. Pharmacol. 289 (2015) 361-370.

Awasthi Y. C. et al. Regulatory roles of glutathione-S-transferases and 4-hydroxynonenal in stress-mediated signaling and toxicity. Free Radical Biology and Medicine 2016.

Kullich W, Fagerer N, Schwann H. Effect of the NSAID nimesulide on the radical scavenger glutathione S-transferase in patients with osteoarthritis of the knee. Curr Med Res Opin 2007;23:1981–1986.

Shaat R. M. et al. Impact of obesity on status of glutathione reductase activity and inflammatory response in patients with knee osteoarthritis. International Journal 2015;3(9):609-616.

Campo G. M. et al. Glycosaminoglycans modulate inflammation and apoptosis in LPS-treated chondrocytes. Journal of cellular biochemistry 2009;106(1):83-92.

Henrotin Y. et al. Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations. Therapeutic advances in musculoskeletal disease 2010;2(6):335-348.


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