Functioning of the antioxidant system of rat tissues poisoned with heavy metals

I. Kalinin, B. Tsudzevich
National Pedagogical Dragomanov University, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


To investigate the functioning of antioxidant system in blood and liver of rats, poisoned of heavy metals (copper sulfate, zinc sulfate, cadmium sulfate and lead nitrate) on lipid peroxidation and on activity of glutathione-dependent enzymes of blood and liver of poisoned rats are shown in this article. It is shown that the introduction of heavy metals in rats leads to an increase in blood and liver TBARS-products and diene conjugates. Under the action of heavy metals decreases the activity of catalase and superoxide dismutase. A reduction in the activity of glutathione peroxidase and glutathione transferase under the influence of high doses of heavy metals. Under the action of heavy metals reduced glutathione content in rat tissues.


Keywords


copper, zinc, cadmium, lead, blood, liver, rats, antioxidant system

Full Text:

PDF>PDF

References


Tsudzevych B.А. Ksenobiotiki: nakopichenna, detoksicatsia ta vividenna z zhivich organismiv [monografiya] / Tsudzevych B.А., Stolar O.B., Kalinin I.V., Yukalo V.G. – Kyiv-Ternopil: Vidavnitstvo TNTU imeni I. Pulyuya; 2012. 384 p.

Antioksidantna sistema zachistu organizmu / I.F. Byelenichev, E.L. Levitsky, Y. I. Gubskiy and others // Sovremennye problemy Toksicologiy. 2002:3:24-31.

Wickens A.P. Ageing and the free radical theory / A.P. Wickens // Respiratory Physiology. 2001:3:379-391.

Funktsionuvanna antioksidantnoy systemy shuriv za diy cadmiyu / S.V. Khyzhnyak, A.A. Prokhorova, V.A. Gryschenko and others // The Ukrainian biochemical journal. 2010:4:105-111.

Khyzhnyak S.V. Klitynni mekhanismy toksichnosti cadmiyu / S.V. Khyzhnyak. – Kyiv: Edition "LAT&K"; 2010. 213 p.

Korzhov V.I. Rol sistemy glutathiona v protsessah detoksikatsii I antioksidantnoy zashchity / V.I. Korzhov, V.N. Zhadan, M.V. Korzhov // Zhurnal AMN Ukrainy. 2007:1:3-20.

Kamyshnikov V.S. Spravochnik po kliniko-biokhimicheskoy laboratornoy diagnostike / V.S. Kamyshnikov. Minsk: Belarus. 2000: 90-128.

Stalnaya Y.D. Sovremennye metody v biokhimii / Y.D. Stalnaya, T.G. Haryshvyly; edited V.N. Orekhovich. – M.: Medicine. 1977:66-68.

Gavrilov V.B. Izmerenie dienovykh conyugatov v plasme krovi po UF poglosheniyu geptanovykh i izopropanolnykh ekstractov / V.B. Gavrilov, A.R. Gavrilov, N.F. Khmara // Laboratornoe delo. 1988:2:60-63.

Kostyuk V.A. prostoy i chuvstvitelnyy metod opredeleniya SOD, osnovannyy na reaktsiy okisleniya kvertsetina / V.A. Kostyuk, A.I. Potapovich, Zh.I, Kovaleva //Voprosy medetsinskoy khimiy.1990:2:88-91.

Koroliuk M.A. Metod opredeleniya activnosti katalazy v biologicheskom materiale / M.A. Koroliuk // Laboratornoe delo. 1988: 2:31-34.

Mannervik B. Glutathione peroxidase / B. Mannervik // Methods in enzymology. Academic Press. 1985:113:490-495.

Vlasova S.N. Aktivnost glutationzavisimych fermentov eritrotsitov pri khronicheskikh zabolevaniyach pecheni u detey / S.N. Vlasova, E.I. Shabunyna, A.I. Pereslehyna // Laboratornoe Delo. 1990:8:1 9-21.

Ellman G.L. Tissue sulfhydryl groups / G.L. Ellman // Archives of Biochemistry and Biophysics. 1959.:1:70-77.

Lowry O.H. Protein measurement with folin phenol reagent / O.H. Lowry, H. J. Rosebroungh, A. L. Farr, R. J. Randall // Journal of Biological Chemistry. 1951:1:265-275.

Kucherenko N.E. Suchasni metody biokhimichnich doslidzhen / N.E. Kucherenko, Y.D. Babenyuk, V.M. Voitsitsky. – Kyiv: Edition Fitosotsiotsentr. 2001:109-152.




DOI: http://dx.doi.org/10.17721/2616_6410.2016.21.5-8

Refbacks

  • There are currently no refbacks.


Лицензия Creative Commons
This journal is available according to the Creative Commons License «Attribution» («Атрибуція») 4.0 Global (CC-BY).