The effect of IgG fraction from blood plasma of patients with acute coronary syndromes on the parameters of primary haemostasis

T. Marynenko, T. Halenova, L. Ostapchenko
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


Coronary heart disease is the most common form of cardiovascular disease, which is one of the leading causes of death and disability worldwide. Today, the search for effector molecules of endogenous origin and investigation of their possible role as new therapeutic targets and biological markers are underway. Among such molecules, special attention should be paid to immunoglobulins G (IgG), which accumulate in the general bloodstream in response to the appearance in the body of atypical and modified proteins, as well as their fragments, which is a natural consequence of activation of inflammatory and hypercoagulation reactions under coronary heart disease. In this study, in experimentsin vitro, we investigated the effect of IgG obtained from the serum of patients with unstable angina and myocardial infarction on platelet aggregation and endothelin-1 and von Willebrand factor secretion by cells in HUVEC culture. We did not observe any effect of IgG obtained from the serum of patients with coronary heart disease, both on platelet activation and on the dynamics of ADP-dependent cell aggregation. However, the addition of IgG to the HUVEC cell culture medium showed an increase in cell secretion of both von Willebrand factor and endothelin-1. High concentrations of von Willebrand factor in the systemic circulation can cause an increase in prothrombotic potential, while the accumulation of endothelin-1 may contribute to the development of hypoxia in both the myocardium and other peripheral tissues. All this can lead to disruption of coagulation processes, and, as a consequence, the development and progression of complications of coronary heart disease. It should be noted that antibodies obtained from the serum of patients with progressive unstable angina had the most pronounced effect. The obtained results reveal new perspectives in the study of the effector properties of antibodies and their role in the pathogenesis of coronary heart disease.

Keywords


Myocardial infarction; unstable angina; coronary heart disease; immunoglobulinsG; platelet aggregation; von Willebrand factor; endothelin-1

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References


Korzh O. M. Current aspects of coronary heart disease diagnosis and treatment. Mizhnarodnyy medychnyy zhurnal. 2020; 1:5-10.

Terenda N.O. Tendentsii ta prohnoz poshyrenosti stenokardii ta infarktu miokarda v Ukraini / N.O. Terenda // Visnyk sotsialnoi hihiieny ta orhanizatsii okhorony zdorovia Ukrainy. – 2015. – № 3 (65). – S.35-40.

Hajar R. Risk Factors for Coronary Artery Disease: Historical Perspectives. Heart Views. 2017;18(3):109-114. doi: 10.4103/HEARTVIEWS. HEARTVIEWS_106_17.

Morange PE, Bickel C, Nicaud V, et al. Haemostatic factors and the risk of cardiovascular death in patients with coronary artery disease: the Athero Genestudy. Arterioscler Thromb Vasc Biol. 2006;26(12):2793-2799. doi: 10.1161/01.ATV.0000249406.92992.0d

Katrii T.,Vovk T.,Kravchenko N., etal. Influence of IgG separated from blood plasma of patients with ischemic stroke on the process platelet's proteins secretion. International Journal of Chemical and Biomolecular Science. 2015;4: 278-283.

Katrii T., Kravchenko N., Vovk T., et al. The influence of immunoglobulin class G from blood plasma of patients with stroke on the activity of some parameters of haemostasis system. Journal of Blood Coagulation and Fibrinolysis. 2016;27(8):876-881.

Katrii T. Savchuk O. Platelets aggregation under influence of IgG separated from the blood plasma of the patients with ischemic stroke. Visnyk KNU imeni Tarasa Shevchenka. Seriia: Problemy rehuliatsii fiziolohichnykh funktsii. 2016;1(20):22-25.

Katrii T.,Raksha N., Halenova T. Effects of IgG from the serum of ischemic stroke patients on hemostasis. Journal of Biological Research. 2021;94:9582. doi:10.4081/jbr.2021.9582.

Meier L.A. BinstadtB.A. The Contribution of Auto antibodies to Inflammatory Cardiovascular Pathology. Front Immunol. 2018;9:911. doi: 10.3389/fimmu.2018.00911.

Received in the editorial 16.09.2021

Received version on 16.10.2021

Signed in the press on 16.10.2021




DOI: http://dx.doi.org/10.17721/1728.2748.2021.87.27-31

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