Influence of salt stress on plants of poplar clone "INRA 353-38" and willow clone "Zhytomyrska-1" in in vitro culture

Yu. Khoma, L. Khudolieieva, N. Kutsokon
Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv; Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv; Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv

Abstract


Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal genotypes that can be planted in saline soils for producing biomass for the bioenergy industry. Therefore, the aim of the study was to investigate the effects of salt stress on poplar plants of clone 'INRA 353-38' (Populus tremula × P. tremuloides) and willow clone 'Zhytomyrska – 1' (Salix sp.) under in vitro culture. For this purpose the plants were cultivated on MS nutrient medium with the addition of sodium chloride in concentrations 25 mM, 50 mM and 100 mM. The control plants were grown on the sodium chloridefree medium. The plant status (with a 4-score scale), the intensity of their growth (by shoot length) and rooting capacity (by the number of roots) were assessed on the 10th and the 30th day of cultivation. The results obtained indicate a high level of sensitivity to sodium chloride of both studied clones under in vitro cultivation. But the willow 'Zhytomyrska – 1' had a higher sensitivity to salt stress comparing to hybrid polar 'ІNRA 353-38' since growth parameters of willow were significantly decreased even under the concentration of sodium chloride 50 mM, and in the case of short term influence (10 days) of the highest concentration of sodium chloride (100 mM) all willow plants terminated their growth and quickly died. The growth parameters of hybrid poplar were declined within a month, mainly under the highest concentration of sodium chloride, but even under such conditions some part of the shoots were able to survive.

Keywords


poplar (Populus), willow (Salix), salt stress, in vitro culture

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References


Blumwald E. Sodium transport and salt tolerance in plants. Curr. Opin. Cell Biol. 2000;12:431-434.

Barcala M., Mattera M., Soliani C., et al. Molecular bases of responses to abiotic stress in trees. Journal of Experimental Botany. 2019; 1-15.

Chen S., Polle A. Salinity tolerance of Populus . Plant Biol. 2010;12: 317 – 33.

FAO & IFAD. Status of the World's Soil Resources (SWSR) // Food and Agriculture Organization of the United Nations and Intergovernmental Technical Panel on Soils .Rome, Italy. 2015:648.

Fischer U., Polle A. Populus responses to abiotic stress. Genetics and genomics of Populus. Springer. 2010; 3:225 – 246.

Flowers T.J., Yeo A.R. Breeding for salinity resistance in crop plants: where next? Aust. J. Plant Physiol.1995;22:875-884

Gu R., Fonseca S., Puskás L., et al. Transcript identification and profiling during salt stress and recovery of Populus euphratica. Tree Physiol. 2004;24:265–276.

Hangs, R. D., Schoenau, J. J., Rees V., et al. Examining the salt tolerance of willow (Salix spp.) bioenergy species for use on salt-affected agricultural lands. Can. J. Plant Sci. 2011; 91: 509–517.

Hasegawa P.M., Bressan R.A., Zhu J.K., et al. Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Physiol. Plant Mol. Biol. 2000; 51:463–499.

Jansson S., Douglas C. Populus: a model system for plant biology. Annu Rev Plant Biol. 2007;58:435 – 58.

Li Y., Su X., Zhang B., Huang Q., et al. Expression of jasmonic ethylene responsive factor gene in transgenic poplar tree leads to increased salt tolerance. Tree Physiol. 2009; 29 (2): 273–279.

Munns R., Tester M. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol. 2008; 59:651-681.

Rees V. Developing a national agroforestry and afforestation network for Canada. Policy Options. 2008; 29:54-57.

Zhang J., Yuan H., Yang Q., et al. The genetic architecture of growth traits in Salix matsudana under salt stress. Hortic Res. 2017; 4:17024.

Sahi C., Singh A., Blumwald E., et al. Beyond osmolytes and transporters: novel plant salt-stress tolerance-related genes from transcriptional profiling data. Physiol. Plant. 2006; 127:1-9

Šerá, B. Salt-tolerant trees usable for Central European cities – Review. Horticultural Science. 2017; 44(1): 43-48.

Teakle N.L., Tyerman S.D. Mechanisms of Cl(-) transport contributing to salt tolerance. Plant Cell Environ. 2010; 33:566-589.

Tester M., Davenport R. Na + tolerance and Na + transport in higher plants. Ann. Bot. 2003; 91:503-527.

Urbańska S., Obrzut P., Ogiela E. Impact of salts from winter road maintenance on selected properties of roadside soils. Infrastructure and ecology of rural areas. 2016; 1521 – 1534.

Balyuk S., Medvedev V., Miroshnichenko V., at al. Ecological condition of soils of Ukraine. Ukrainian Geographical Journal. 2012; 38-42.

Kosakivska I., Golovyanko I. Adaptation of plants: biosynthesis and functions of stress proteins. Ukrainian phytocenological collection. 2006; 3-17.

Kutsokon N. The main directions of genetic transformation of the genus Populus. Cytology and Genetics. 2011; 6:67-78.

Kutsokon N., Rakhmetov D., Khudolieieva L. et al. Growth characteristics and energy productivity of poplars and willows under short rotation planting for the first vegetation year. Biological systems. 2017; 9 (2):238-246.

Khudoleeva L., Kutsokon N. Comparison of salt resistance of representatives of the genera Populus and Salix in vitro. Science Rise: Biological Science. 2018; 2 (11):35-38.

Received: 7.10.2020

Revised: 9.11.2020

Signed for the press: 9.11.2020




DOI: http://dx.doi.org/10.17721/1728_2748.2020.83.43-49

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