Purification of microalgae crops of ACKU collection from fungal contaminants

V. Petlyovana, Minglei Chen
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


The work is focused to the selection of the best purification methods of microalgae strains from ACKU collection (Algae Culture Collection of Kyiv University) from contamination by microscopic fungi. The screening of microalgae culture collection ACKU (Algae Culture Collection of Kyiv University) is deal. Contamination of some microalgae strains by fungi of Cladosporium Link, Alternaria Nees, and Monilia Bonord genera was detected. The following strains were selected for the experiment: ACKU 139-02 (Klebsormidium flaccidum (Kütz.) PC Silva, Mattox et Blackwell), ACKU 293-04 (Acutodesmus obliquus (Turpin) P. Tsarenko), ACKU 364-04 (cf. Chlorosarcinopsis dissociata Herndon), ACKU 599-06 (Klebsormidium nitens Menegh. in Kützing), ACKU 600-06 (Klebsormidium flaccidum (Kütz.) PC Silva, Mattox et Blackwell) и ACKU 1056 (Desmodesmus abundans (Kirchn.) E. Hegew.). The effect of carbendazim (Methylbenzimidazol-2-ylcarbamate), Antibiotic Antimycotic Solution (penicillin – 10,000 IU, streptomycin – 10 mg, amphotericin B – 25 μg) and Nuosept BMc 422 on fungi-contaminants of green algal culture strains with different morphological structure (coccoid and filamentous) was studied. Experimental concentrations of substances: carbendazim – 0.005%, Antibiotic Antimycotic Solution – 1%, Nuosept BMc 422 – 0.05% and 0.2%. Was shown that Antibiotic-Antimycotic Solution and Nuosept BMc-422 at the selected concentrations were not effective for purifying of green algae strains from significant contamination by microscopic fungi. It was found that the carbendazim solution is effective in the purification of cocoid green algae strains from contamination by microscopic fungi Cladosporium cladosporioides (Fresen.) G.A. de Vries). As a result, an axenic culture was obtained for the ACKU strain No.293-04 (A. obliquus).

Keywords


strain, ACKU, cultivation, microscopic green algae, Chlorophyta, contamination, Сarbendazim, Antibiotic Antimycotic Solution, Nuosept BMc 422

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References


Kostikov I.Iu., Demchenko E.M., Berezovska M.A. Kolektsiia kultur vodorostei Kyivskoho natsionalnoho universytetu imeni Tarasa Hryhorovy cha Shevchenka. Kataloh shtamiv (2008 r). //Chornomorsk. bot. zhurn. 2009; 5 (1): 37-79.

Borowitzka MA. Micro algaeas source of pharmaceuticals and other biologically active compounds // J. Appl. Phycol. 1995; 7: 3-15.

Larkum A.W., Ross I.L., Kruse O., Hankamer B. Selection, breeding and engineering of micro algae for bioenergy and biofuel production. Trends Biotechnol. 2012; 30: 198-205.

Mahan, K.M., Odom O.W., Herrin D.L. Controlling fungal contaminationin Chlamydomonas reinhardtii cultures. Biotechniques. 2005; 39:457-458.

Wasanasathian A., Peng C.A. Algal photobioreactor for production of lutein and zeaxanthin.In: Shang-Tian Yangeditor. Bioprocessing for ValueAdded Products from Renewable Resources. Elsevier. – 2007. p. 491-505.

Received: 2.10.2020

Revised: 2.11.2020

Signed for the press: 2.11.2020




DOI: http://dx.doi.org/10.17721/1728_2748.2020.83.29-32

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