Proteolytic activity of rat blood plasma and liver under chronic alcohol intoxication

A. Serbin, T. Koval, O. Kharchenko
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv


Today, the problem of alcohol abuse is quite relevant around the world. According to the World Health Organization, alcohol abuse is one of the
three causes of premature death and is one of the main etiological factors that that cause the development of chronic diseases of the liver and other organs. The severity of clinical manifestations and the prognosis of further dynamics of ongoing alcohol intoxication can be investigated by studying the composition of proteins in tissues, as it is known that this pathology has a pronounced protein dystrophy and active proteolysis. Although much is known today about the effects of alcohol on proteolytic systems, many questions still need to be explored, as proteomic studies can be used to find biomarkers and therapeutic targets for ethanol abuse. The aim of the study was: to investigate the proteolytic activity of blood plasma and liver of rats in chronic alcohol intoxication at 11 and 21 days of the experiment. The model of chronic alcohol intoxication was modeled on male rats – weight 180-200g by intragastric administration of 30% ethyl alcohol solution for 10 days on an empty stomach, at the rate of 2 ml per 100 g of animal weight. Total proteolytic activity, activity of metalloproteinases and serine proteinases were determined by the method of determining caseinolytic activity. The protein concentration was determined by the Bradford method. Statistical processing of the study results was performed by conventional
methods of variation statistics. It was found that on the 11th day from the beginning of ethanol administration to rats, there was an increase in total proteolytic activity and activity of metetalloproteinases in blood plasma and liver.

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Torrente M., Freeman W., Vrana K. Protein biomarkers of alcol abuse. Expert Rev Proteomic. 2012; 9(4): 425–36.

Souza-Smith F., Lang C., Nagy L., Bailey S. Physiological processes underlying organ injury in alcohol abuse. Am J Physiol Endocrinol Metab. 2016; 311(3): 605–19.

Kimball S. R., Lang C. H. Mechanisms Underlying Muscle Protein Imbalance Induced by Alcohol. Annu Rev Nutr. 2018 Aug 21; 38: 197–217.

Khalilov M. K., Zakikhorzhaev S. Y. On the characterization of certain pathochemical changes in the blood, liver and brain tissues during experimental alcohol intoxication. Problems of the alcoholism clinic: Соl sci works, Tashkent. 1983: 38–41.

Menshikov V. Laboratory research methods in the clinic. M. : Medicine, 1987. 310 s.

Huang X. J., Choi Y. K., Im H. S. et al. Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques. Sensors (Basel). 2006 Jul 31; 6 (7): 756–82.

Hummel В. С. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Canadian Journal of Biochemistry and Physiology. 1959; 37 (12): 1393–9.

Tarasova O., Mazurchik N., Ogurtsov P., Moiseev V. Modern laboratory markers of alcohol consumption. Clinical pharmacology and therapy. 2007; 1: 1–5.

Topic A., Djukic M. Diagnostic characteristics and application of alcohol biomarkers. Clin Lab. 2013; 59(3–4): 233–45.

Jastrzębska I., Zwolak A., Szczyrek M. et al. Biomarkers of alcohol misuse: recent advances and future prospects. Prz Gastroenterol. 2016; 11 (2): 78–89.

Kharchenko O. Content of blood plasma trypsin-like enzymes and molecules of average mass as pontetial markers of the chronic alcocholic intoxication. Bulletin of Taras Shevchenko National University of Kyiv – Biology 2015; 69 (1): 61–4.

Casini A., Ceni E., Salzano R. et al. Acetaldehyde regulates the gene expression of matrix-metalloproteinase-1 and -2 in human fat-storing cells. Life Sci. 1994; 55: 1311–6.

Panchenko L., Terebilina N., Pyrozhkov S. et al. Serum markers of fibrosis and endothelial dysfunction in patients with alcoholism, with varying degrees of liver fibrosis. Patol Fiziol Eksp Ter. 2015; 55(3): 18–27.

Hodos O., Gidranovich G., Sachek M. Features of proteolysis in rat brain tissue in chronic alcohol intoxication. Bulletin of Vitebsk State Medical University. 2008; 7(1): 24–31.


Received: 10.04.2020

Revised: 11.05.2020

Signed for press: 11.05.2020



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