Suppression of gastric ulcers formation under immobilization water-immersion stress by preliminary transplantating the multipotent stromal cells

Y.-M. Semenova, I. Nikolsky, L. Ostapchenko
State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of Ukraine, Kyiv; State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of Ukraine, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


To investigate the effect of pre-transplantation of multipotent stromal cells (MSCs) of bone marrow on gastric ulcer formation and the state of
the immune system in conditions of acute and prolonged stress. Wistar rats reproduced immobilizing water-immersion stress of 2 types: acute and prolonged. Investigated the number and area of stress ulcers, thymus and spleen, as well as hematologic parameters, proliferative and cytotoxic activity of peripheral blood mononuclear cells, splenocytes and cells of lymph nodes, determined the absorption activity of neutrophils. With prolonged stress as a result of MSC transplantation, the number and area of ulcers significantly decreased, indicating the adaptive protective effect of cells. With acute stress, the introduction of MSC had virtually no effect on ulcer formation. With prolonged stress, there was a decrease in thymus, spleen and leukocyte counts in the blood. Under the influence of transplanted MSCs, the number of all mobilized cells was normalized with the exception of lymphocytes. The natural cytotoxicity and proliferative activity of splenocytes, cells of lymph nodes and peripheral blood in acute and prolonged stress as a result of the introduction of MSC did not change significantly. The introduction of bone marrow MSС 24 h before the last reproduction of stress responses in the model of prolonged stress significantly reduced the number and area of ulcers, which generally indicates the anti-ulcer effect of cells, and normalized the stress-induced quantitative cellular changes in the immune system. Transplantation of bone marrow MSCs to rats prior to reproduction of stress enhances the adaptive antistress mechanisms that develop during prolonged stress, leading to suppression of gastric ulcer formation and significantly altering immune system activity. It can be assumed that one of the mechanisms of action on the body of MSCs is to promote the formation of adaptive responses.

Keywords


water-immersion cold stress, immune system, multipotent stromal cells, transplantation

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References


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Received: 07.10.2019

Revised: 08.11.2019

Signed for the press: 08.11.2019




DOI: http://dx.doi.org/10.17721/1728_2748.2019.79.72-78

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