Effect of bioactive extracts with high cytokinin content from micelial biomass of Hericium coralloides and Fomitopsis officinalis on tumor cells in vitro

N. Vedenicheva, G. Al-Maali, N. Bisko, I. Kosakivska, L. Garmanchuk, L. Ostapchenko
M. G. Kholodny Institute of Botany of NAS of Ukraine, Kyiv; M. G. Kholodny Institute of Botany of NAS of Ukraine, Kyiv; M. G. Kholodny Institute of Botany of NAS of Ukraine, Kyiv; M. G. Kholodny Institute of Botany of NAS of Ukraine, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv


Phytohormones cytokinins are known to promote cell division in plants. Contrary, in animal's and human's tissues they induce apoptosis and block the cell cycle of a wide spectrum of tumour cells. Therapeutic effects of cytokinins, specifically their anticancer and immunomodulatory actions are similar to those of medicinal mushrooms. We detected cytokinins in mycelial biomass of two species of medicinal mushrooms growing in vitro (Fomitopsis officinalis strain 5004 and Hericium coralloides strain 2332) using HPLC-MS. Trans-zeatin, zeatin riboside, zeatin-O-glucoside and isopentenyladenine were found. Crude extracts and purified cytokinin fractions from mycelial biomass were tested on the growth and development of cultures of tumor cells lines: Hela (MTT-assay), T24/83 (viability and level apoptotic cells) and HepG2 (consumption of glucose). The effect of cytokinin fraction from mycelial biomass of Fomitopsis officinalis on pathogenic cells was higher compared to Hericium coralloides one. The data obtained revealed a higher cytotoxic/cytostatic effect of the purified cytokinin fractions in comparison with crude methanolic extracts; also higher apoptotic index was found. Under the influence of the test agents the intensification of glucose uptake into cells was observed. This indicator was higher for crude mushroom mycelium extracts, whereas under the action of purified fractions the glucose uptake rate was lower, thus decreased glycolysis level was recorded. Also, the effect of both crude extract and purified fraction from H. coralloides mycelial biomass on glucose uptake in the conditioned medium was lower against F. officinalis.These results confirm the assumption that biologically active substances of medicinal mushrooms with high pharmacological potential include cytokinins.

*Investigations were supported by grant 8B "Research of antitumor properties of biologically active substances of the cytokinin
nature from mycelial biomass of medicinal basidial mushrooms".


medicinal mushrooms, mycelial biomass, cytokinins, HepG2, T-24/83 and Hela cells

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Received: 02.10.2019

Revised: 04.11.2019

Singed for press: 04.11.2019

DOI: http://dx.doi.org/10.17721/1728_2748.2019.79.31-37


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