Pathogen-induced callose and phenolic compounds accumulation in winter wheat seedlings

O. Boboshko, O. Panyuta, O. Artemenko, N. Taran, V. Emelyanov
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Institute of Cell Biology and Genetic Engineering, Kyiv

Abstract


Callose and/or phenolic compounds accumulation, which leads to the plant cell wall reinforcement in the places of the host plant and pathogen contact, is one of the first plants reactions on to the pathogen. The local protective reaction causes systemic protective mechanisms that cover the entire plant organism. The purpose of the work was determination of callose and phenolic compounds content in winter wheat seedlings of Myronivska 808 and Renan varieties as constitutional and induced by the eyespot causal agent in the range of their titres active concentrations and comparison of these substances accumulation in time. Callose determination was carried according to the modified Kauss et al. method. Phenoliccompounds quantificationassaywas basedon Folin-Ciocalteu method. The content of constitutional and pathogen-induced callose and phenolic compounds in the winter wheat seedlings of Myronivska 808 and Renan varieties was determined. The higher content of constitutional callose and phenolic compounds at the initial stages of vegetation was established in the wheat seedlings of relatively resistant variety Renan than in the seedlings of the susceptible variety Myronivska 808, which is related with genetically determined resistance of this variety to the eyespot causal agent. More intensive pathogen-induced accumulation of callose was observed in wheat seedlings of Myronivska 808 variety, but this did not affect the overall effectiveness of protection against pathogen. The highest pathogen-induced callose accumulation in wheat seedlings of a relatively resistant variety Renan was under inoculum concentration 104 CFU/ml and in seedlings of the susceptible variety Myronivska 808 – 102 CFU/ml. It has been shown that the higher content of constitutional callose and phenolic compounds and their intensive accumulation under infection in the wheat seedlings of Renan variety, is a determinative factor of the resistance to the eyespot causal agent.

Keywords


callose, phenolic compounds, wheat, eyespotcausal agent

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References


Emelyanov V., Kravchuk J., Poliakovskiy S., Dmitriev O. Callose deposition in the processing of the cells of tomato (Lycopersicon esculentum L.) biotic elicitors, Cytology and Genetics, 2008, vol. 42, № 2, pp. 21–28.

Prigogine I., Kondepudi D. Modern thermodynamics. From Heat Engines to Dissipative Structures, M .: Mir, 2002 – 462 p.

Cle C., Hill L., Niggeweg R., Martin, C., Guisez Y., Prinsen E., Jansen M. Modulation of chlorogenic acid biosynthesis in Solanum lycopersicum; consequences for phenolic accumulation and UV-tolerance,Phytochemistry, 2008, vol. 69, № 11, pp. 2149–2156.

Zeyen R., Carver T., Lyngkjаr M. Epidermal cell papillae. The Powdery Mildews: a comprehensive treatise. MN, USA: APS Press; 2002. pp. 107–125.

Ferrazzano G., Amato I., Ingenito A., Natale A., Pollio A. Anticariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea), Fitoterapia, 2009, vol. 80, № 20, pp. 255–262.

Karou D., Dicko M., Simpore J., Traore A. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso, African Journal of Biotechnology, 2005, vol. 4, № 8, pp. 823–828.

Conrath U., Beckers G., Flors V., García-Agustín P., Jakab G., Mauch F., Newman M., Pieterse C., Poinssot B., Pozo M., Pugin A., Schaffrath U., Ton J., Wendehenne D., Zimmerli L., Mauch-Mani B. Priming: getting ready for battle, Mol. Plant-MicrobeInteract, 2006, vol. 19, № 10, pp. 1062–1071.

Pastor V., Luna E., Mauch-Mani B., Ton J., Flors V. Primed plants do not forget. Environmental and Experimental Botany, 2012, vol. 94, pp. 46–56.

Newman M., Dow J., Molinaro A., Parrilli M. Priming induction and modulation of plant defense responses by bacteria lipopolysaccharides, Journal of Endotoxin Research, 2007, vol.13, pp. 69–84.

Zipfel C. Pattern-recognition receptors in plantinnateimmunity, Current Opinion in Immunology, 2008, vol.20, № 1, pp.10–16.

Nicaise V., Rouх M., Zipfel C. Recent advances in PAMP-triggered immunity against bacteria: pattern recogniti on receptorswatch over and raise the alarm, Plant Physiology, 2009, vol. 150, № 4, pp.1638–1647.

Plotnikov L. Cytological, molecular and genetic bases of plant species immunity to fungal pathogens, Mycology and Phytopathology, 2008, vol. 42, № 5., pp. 393–410.

Chowdhury J., Henderson M., Schweizer P., Burton R., Fincher G., Little A. Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp. Hordei, New Phytologist, 2014, vol. 3, № 204, pp.650–660.

Underwood W. The Plant Cell Wall: A Dynamic Barrier Against Pathogen Invasion, Front. Plant. Sci., 2012, vol. 3, № 8, pp. 483–593.

Wei L. Advances and prospects in wheat eyespot research: Contribution from genetics and molecular tools. Journal of Phytopathology, 2011.

Panyuta O., Belava V., Taran N. Method of infection to assess the stability of winter wheat to the pathogen tserkosporelozu, UA Patent no. 389960, Ukraine, A01N 1/04, Bull. № 9, 2014.

Kauss H., Jeblick W., Domard A. The degrees of polimerization and N-acetylation of chitosan determine its ability to elicit callose formation in suspension cells and protoplasts of Catharanthus roseus, Planta, 1989, vol. 178, issue 3, pp. 385–392.

Kim K., Tsao R., Yang R., Cui S. Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions, Food Chemistry, vol. 95, issue 3, pp. 466–473.

Lysenko N., Prudnikova E., Khilkova N., Tchekalin E. Features of physiological and biochemical processes of spring wheat plants after using fungicide, Scientific notes of Orel state university. Series: Natural, technical and medical sciences, 2012, № 6-1, pp. 385–388.

Received in the editorial: 17.09.2018

Received a revised version: 18.10.2018

Signed in the press: 18.10.2018


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