Oxidative modification of proteins in serum of rats under conditions of carrageenan-induced inflammation of a back limb and prolonged prophylactic administration of chondroitin sulfate

O. Blokhina, O. Korotkiy, L. Kot, D. Negray, K. Dvorshchenko
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv


The aim of the work was to investigate the preventive effect of Chondroitin Sulfate on the content of products of oxidative modification of proteins and the level of sulfhydryl groups in blood serum of rats at local inflammation of the hind limb. The studies were conducted on white non-linear, sexually mature male rats weighing 180–240 g, in compliance with the general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – control: animals sub-planar injected 0,1 ml of 0,9 % NaCl solution into the posterior right limb. The second group – animals received a therapeutic dose of 3 mg x kg-1 chondroitin sulfate daily for 28 days daily. The third group – animals were infused intramuscularly with 0,1 ml of 0,9 % NaCl solution in the posterior right limb for 28 days and for 29 days inflammatory edema of the limb was stimulated (animals were sub-planar injected with 0,1 ml of 1 % carrageenan solution to the posterior right limb ) The fourth group – for 28 days rats were daily intramuscularly injected with a therapeutic dose of 3 mg x kg-1 chondroitin sulfate, after which on 29th day, inflammatory edema of the limb was stimulated. Animals were killed 3 hours after injection of carrageenan solution according to the protocol of the ethical committee, and then blood sampling for further research was quickly taken. The total number of animals involved in experimental studies was 40 individuals. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-dinitrophenylhydrazine. The level of total, protein-bound and non-protein sulfhydryl (SH) -groups was measured by the Elman method. It has been established that with carrageenan-induced inflammation of the posterior limb, the content of the products of oxidative modification of proteins increases and the content of sulfhydryl groups decreases in the serum. It was shown that the prophylactic administration of chondroitin sulfate based drug on animals with carrageenan-induced inflammation restored the abovementioned parameters.


acute inflammation of the limb, chondroitin sulfate, oxidative modification of proteins, blood serum

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Man G.S., Mologhianu G. Osteoarthritis pathogenesis – a complex process that involves the entire joint // J. Med. Life. – 2014. – Vol. 7, №1. – P. 37-41. Available from: http://www.ncbi.nlm.nih.gov

Drevet S., Gavazzi G., Grange L. et al. Reactive oxygen species and NADPH oxidase 4 involvement in osteoarthritis // Exp. Gerontol. – 2018. – Vol. 111. – P. 107-117. Available from: http://www.ncbi.nlm.nih.gov

Bishnoi M., Jain A., Hurkat P., Jain S.K. Chondroitin sulphate: a focus on osteoarthritis // Glycoconj. J. – 2016. – Vol. 33, №5. – P. 693-705. Available from: http://www.ncbi.nlm.nih.gov

Morris C.J. Carrageenan-induced paw edema in the rat and mouse // Methods Mol. Biol. – 2003. – Vol. 225. – P. 115–121. Available from: http://link.springer.com/protocol/10.1385/1-59259-374-7%3A115

Дубинина Е.Е., Бурмистров С.О., Ходов Д.А., Поротов И.Г. Окислительные модификации белков сыворотки крови человека, метод ее определения // Вопросы медицинской химии. – 1995. – № 1. – С. 24–26. Available from: http://pbmc.ibmc.msk.ru/index.php/ru/article/PBMC-1995-411-24-ru

Ellman G. Tissue sulfhydryl groups / G. Ellman // Arch. Biochem. Biophys. – 1959. – Vol. 82, №1. – P. 70–77. Available from: http://aufsi.auburn.edu/recommendedmethods/01B06.pdf

Реброва О.Ю. Статистический анализ медицинских данных. – М.: Информполиграф, 2002. – 305 с. Available from: http://www.twirpx.com/file/1440496/

Dahl J.U., Gray M.J., Jakob U. Protein quality control under oxidative stress conditions // J. Mol. Biol. 2015. – Vol. 427, №7. – P. 1549-1563. Available from: http://www.ncbi.nlm.nih.gov

Pajares M., Jiménez-Moreno N., Dias I.H. et al. Redox control of protein degradation // Redox. Biol. – 2015. – Vol. 6. – P. 409-420. Available from: http://www.ncbi.nlm.nih.gov

Breusing N., Grune T. Biomarkers of protein oxidation from a chemical, biological and medical point of view // Exp. Gerontol. – 2010. – Vol. 45, №10. – P. 733-737. Available from: http://www.ncbi.nlm.nih.gov

Vázquez-Torres A. Redox active thiol sensors of oxidative and nitrosative stress //Antioxid. Redox. Signal. – 2012. – Vol. 17, №9. – P. 12011214. Available from: http://www.ncbi.nlm.nih.gov

Domingues R.M., Domingues P., Melo T. et al. Lipoxidation adducts with peptides and proteins: deleterious modifications or signaling mechanisms? // J. Proteomics. – 2013. – Vol. 92. – P. 110-131. Available from: http://www.ncbi.nlm.nih.gov

Meyer A. Glutathione homeostasis and redox-regulation by sulfhydryl groups / A. Meyer, R. Hell // Photosynth. Res. – 2005. – Vol. 86. – P. 435–457. Available from: http://www.ncbi.nlm.nih.gov

Davies M.J. Protein oxidation and peroxidation // Biochem. J. – 2016. – Vol. 473, №7. – P. 805-825. Available from: http://www.ncbi.nlm.nih.gov

Valko M., Leibfritz D., Moncol J. et al. Free radicals and antioxidants in normal physiological functions and human disease // Int. J. Biochem. Cell Biol.– 2007.– Vol. 39.– P. 44–84. Available from: http://www.ncbi.nlm.nih.gov

Martel-Pelletier J., Farran A., Montell E. et al. Discrepancies in composition and biological effects of different formulations of chondroitin sulfate // Molecules. – 2015. – Vol. 20, №3. – P. 4277-4289. Available from: http://www.ncbi.nlm.nih.gov

Received in the editorial: 14.09.2018

Received a revised version: 15.10.2018

Signed in the press: 15.10.2018


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