Determination of xanthones in plants and the nutrient medium under in vitro cultivation conditions

A. Revutska, V. Belava, A. Golubenko, N. Taran
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv

Abstract


In recent years, xanthones have received considerable attention from scientists due to their biological activity: anticarcinogenic, antiviral, antibacterial, antioxidant, anti-inflammatory and other properties.Therefore they are useful for prevention and treatment of different diseases:cancer, Alzheimer's and Parkinson's disease, cardiovascular disorders, diabetes, etc.  Extracts of different species of plants containing xanthones are components of chemotherapeutic and other medical drugs. In order to find the most sensitive and environmentally safe method of quantitative determination of xanthones in the plant material and the nutrient medium, known methods were tested and selected for the prototype Vyisochina G. I. et al., 2011 method, which uses ethanol as an extractor. As the plant material we used plants of different species that were grown under in vitro cultivation conditions on the agarized nutrient medium. This agarized nutrient medium was also used for the xanthone content analysis. Based on the performed research, modifications of the method for determining the content of xanthones were adapted to the in vitro conditions, which detail the specificity of extraction and quantitative calculation of the xanthone content in plant explants. Our own method of determination of these compounds in the agarized nutrient medium was developed as well. The method, that we proposed, will significantly speed up the process of xanthone detecting and will also increase their yield in biotechnological processes for obtaining the pharmacologically valuable secondary metabolites of phenolic nature.

Keywords


xanthones, chromatography, spectrophotometry, in vitro, plants, nutrient medium

Full Text:

PDF>PDF

References


Cruz I., Puthongking P., Sara Cravo S. [et al.]. Xanthone and Flavone Derivatives as Dual Agents with Acetylcholinesterase Inhibition and Antioxidant Activity as Potential Anti-Alzheimer Agents. J of Chemistry. 2017; Article ID 8587260: 16 p.doi: 10.1155/2017/8587260.

Inoue T., Kainuma M., Baba K. [etal.].Garcinia subelliptica Merr. (Fukugi): A multipurpose coastal tree with promising medicinal properties: A review on Fukugi. J Intercult Ethnopharmacology. 2017; 6 (1): 121-127. doi: 10.5455/jice.20161229060034.

Krstić‑ Milošević D., Janković T., Uzelac.B., Vinterhalter D., Vinterhalter B. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentianadinarica. Plant Cell Tiss Organ Cult. 2017; 130: 631-640. doi: 10.1007/s11240-017-1252-1

Le Pogam P., Boustie J. Xanthones of Lichen Source: A 2016 Update Molecules.2016; 21: 30 p. doi: 10.3390/molecules 21030294.

Li X., Kim J., Park S., Zhao S. [et al.]. Comparative analysis of flavonoids and polar metabolite profiling of Tanno-original and Tanno-high rutin buck wheat. J Agric Food Chem. 2014; 62(12): 2701-2708. doi: 10.1021/jf4049534.

Mirzaeea F., Hosseini A., Jouybaria H. [et al.]. Medicinal, biological and phytochemical properties of Gentiana species. J of Traditional and Complementary Medicine. 2017;

(4): 400-408. doi: 10.1016/ j.jtcme.2016.12.013. 7. Ruan J., Zheng C., Liu Y. [et al.]. Chemical and Biological Research on Herbal Medicines Rich in Xanthones. Molecules. 2017; 22 (1698): 19 р. doi: 10.3390/molecules22101698.

Siti M., Ash Shiddiqi Q. [et al.]. Subcritical Water Extraction of Xanthone from Mangosteen (Garcinia Mangostana Linn) Pericarp. J Adv Chem Eng. 2014; 5(1): 6 р. doi:10.4172/2090-4568.1000117

Wezeman Т., Bräse S ., Masters K. Xanthone dimers: a compound family which is both common and privileged. Nat. Prod. Rep.2015; 32 (1): 6-28. doi: 10.1039/c4np00050a.

Xu С., Wang B., Pu Y. [et al.].Advances in extraction and analysis of phenolic compounds from plant materials. Chin. JNat Med. 2017; 15(10): 721-731. doi: 10.1016/S1875-5364(17)30103-6.

Zubrická D., Mišianiková A., Henzelyová J [et al.]. Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity against Candida albicans.Plant Cell Reports. 2015; 34(11):1953-62. doi: 10.1007/s00299-015-1842-5.

Vyisochina G., Kukushkina T. Biologicheski aktivnyie veschestva nekotoryih vidov roda Hedysarum L. Him rastsyirya. 2011; 4: 251258.Russian. 13. Dospehov B. Metodika polevogo opyita. M.: Agropromizdat.; 1985. 350 p. Russian.

Krivut B., Fedyunina N., Kocherga S., Rusakova S. Spektrofotometricheskoe opredelenie mangiferina. Him.prirodnyih soedineniy. 1976; 1: 44-46. Russian.

Mihaylova T., Shults E., Tahnaeva L. [etal.] Biologicheskaya aktivnost fenolnyih soedineniy, vyidelennyih iz Galenii rogatoy (Haleniacorniculata (L.) Cornaz). Him. v interesah ustoychivogo razvitiya. 2005; 13: 411-415. Russian.

Munhzhargal N., Zibareva L., Ebel A. Vidu semeystva Gentianaceae – perspektivnyie istochniki ekdisteroidov, ksantonov i iridoidov. Bioraznoobrazie, problemyi ekologii gornogo Altaya i sopredelnyih regionov: nastoyaschee, proshloe, buduschee: mater. mezhd. konf. GornoAltaysk. 2008; 9.; Available from: e-lib.gasu.ru/konf/biodiversity /2008/2/28.pdf (accesed,September,2015). Russian.

Revutska A., Belava V., Golubenko A., Taran N. [Content of phenolic compounds in Acoruscalamus L. tissue culture and nutrient culture medium under in vitro conditions]. The Bulletin of Ukr. Society of Gen. and Breeders 2016;14 (2): 210-215. Ukrainian.

Revutska A., Belava V., Golubenko A., Taran N., editors. Eko biotekhnolohichni pidkhody dlia otrymannia in vitro ksantoniv– farmakolohichnykh spoluk. "Ekolohiia ta ekolohichna bezpeka: Materialy naukovo-praktychnoi konferentsii vseukrainskoho studentskoho konkursu".; 2016 March 16-18.; Poltava, Ukraine. Poltava: PoltNTU.; 2016. p. 77. Ukrainian.

Tarasova N., Hlebnikova L., Kaygina E., Lyashevskaya N. Otsenka kolichestvennogo soderzhaniya iridoidov i ksantonov v Gorechavke krupnolistnoy (G. macrophyllaPall.). FEN-NAUKA. 2012; 15 (12): 810. Russian.

Tozhiboev M., Botirov E., Usmanova G. Ksantonyi i flavonoidyi Gentianaalgida Pall. Him.rastitelnogo syirya. 2010; 3: 129-133. Russian.

Received in the editorial: 03.10.2018

Received a revised version: 04.10.2018

Signed in the press: 04.10.2018


Refbacks

  • There are currently no refbacks.


Лицензия Creative Commons
This journal is available according to the Creative Commons License «Attribution» («Атрибуція») 4.0 Global (CC-BY).