Functional characteristics of circulating phagocytes in rats with C6 glioma

Y. Hurmach, M. Rudyk, V. Svyatetska, L. Skivka
Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv; Taras Shevchenko National University of Kyiv, Kyiv

Abstract


Gliomas, tumors of the central nervous system, are a heterogeneous group of neoplasms of neuroectodermal origin. Gliomas are the most common primary brain tumors and cmprise about 80% of all malignant neoplasms of this location. The growth of malignant glioma is accompanied by the development of local and systemic immunosuppression that is a distinctive characteristics of the disease. At the early stages of tumor growth, glioma cells secrete numerous chemokines, cytokines and other biologically active mediators that contribute to the infiltration of tumor tissue by immune cells: resident microglial cells, as well as monocytes, granulocytes, T-cells, B-cells etc. Tumor-associated phagocytes comprise most significant part of the immunocytes in the microenvironment of malignant gliomas. These cells play a leading role in the development of local immunosuppression. The triggers of systemic immunosuppression, as well as the relationship between local and systemic immunosuppression is virtually unexplored and requires an in-depth study of the functional characteristics of circulating cells of the immune system. The aim of this work was to determine the phenotypic and functional characteristics of circulating phagocytes in rats with C6 glioma. Metabolic and phenotypic indices of circulating phagocytes were examined at different time point of tumor growth. It has been established that the late stages of C6 glioma growth in rats are associated with changes in the functional characteristics of peripheral blood mono- and polymorphonuclear phagocytes: a decrease in oxidative metabolism, an increase in phagocytic activity along with an increase CD206 expression. Taken together these alteration are characteristic for alternatively activated phagocytes that can participate in the development of glioma-associated systemic immunosupression. Keywords.

Keywords


circulating phagocytes, glioma, reactive oxygen species, phagocytosis

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References


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Received in the editorial: 30.08.2018

Received a revised version: 1.10.2018

Signed in the press: 1.10.2018


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