The influence of cadmium and essential nanometals on the physiological And biochemical parameters of lettuce plants

I. Khomenko, O. Kosyk, N. Taran
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv; ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv

Abstract


Due to the aim the effect of pre-sowing treatment with a nonionic colloidal solution of the mix of copper, zinc, manganese and iron nanoparticles, cadmium ions and their combined action on the physiological and biochemical indices: the content of lipid peroxidation products and balance of photosynthetic pigments in green Lolo and red Lolo Ross varieties of Lactuca sativa L. was investigated. Spectrophotometric methods of investigating the content of peroxides, thiobarbiturate-active products (with use of the modified Kumar and Knowles method), as well as the content of the main photosynthetic pigments (chlorophyll a, b, a/b ratio, and the carotenoid content) with the Wintermans, de Mots calculations application were used. Due to the results, a decrease in the content of peroxides and growth of malonic dialdehyde was observed for most treatment variants of Lolo. The opposite tendency was observed for the Ross variety (the initial growth of these parameters under the nanoparticles influence decreased to the control during the fourteen-day exposure). The decrease of chlorophyll content was observed In the Lolo variety under the essential metal nanoparticles influence, in contrast to the action of cadmium.For the Ross variety, a significant reduction of both chlorophyll and carotenoid contents in all treatment types during exposure was calculated.The a/b-index for Lolo plants has significantly increased in variants of cadmium influence, for Ross plants the divergence of the influence of all treatment variants has been marked. As a result, the treatment with a mixture of essential nanometals showed additive stress-loading functions. It was observed in a growth of the level of lipid peroxidation products and the reduction of the photosynthetic pigment biomarkers in both varieties during the 0,1 mM cadmium exposure. The Lolo Ross variety showed a higher variability of results under different treatment, compared to the Lolo variety.


Keywords


nanoparticles of essential metals, lipoperoxidation products, pigment complex, Lactuca sativa L.

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References


Azzi V., Kazpard V., Lartiges B. et al. Trace metals in phosphate fertilizers used in Eastern Mediterranean countries. Clean Air Soil Water. 2017 Jan 13;45(1). Epub Sept 2016. doi:10.1002/clen.201500988.

Juárez-Maldonado A., González-Morales S., Cabrera-De la Fuente M. et al. Nanometals as Promoters of Nutraceutical Quality in Crop Plants. Impact of Nanoscience in the Food Industry. 2018;277-310. doi:10.1016/B978-0-12-811441-4.00010-8.

Karimi J, Mohsenzadeh S. Physiological Effects of Silver Nanoparticles and Silver Nitrate Toxicity in Triticum aestivum. Iranian Journal of Science and Technology. 2017;41(1):111–120. doi:10.1007/s40995-017-0200-6.

Konotop Ye., Kovalenko M., Ulynets V. et al. Phytotoxicity of Colloidal Solutions of Metal-Containing Nanoparticles. Cytology and Genetics. 2014;48(2):37–42.

Frantiichuk V., Kovalenko M., Honchar L. et al. Vplyv neionnoho koloidnoho rozchynu nanochastok metaliv na rist i rozvytok ozymoi pshenytsi. Zbirnyk naukovykh prats [Instytutu bioenerhetychnykh kultur itsukrovykh buriakiv]. 2012;14:119-123. Ukrainian.

Liu R., Zhang H., Lal R. Effects of Stabilized Nanoparticles of Copper, Zinc, Manganese, and Iron Oxides in Low Concentrations on Lettuce (Lactuca sativa) Seed Germination: Nanotoxicants or Nanonutrients? Water Air Soil Pollut. 2016;227:42. doi:10.1007/s11270-015-2738-2.

Smirnov OE, Taran NYu, Kosyan AM, et al. Challenging action of low-dose aluminium on physiological condition of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) plants. The Journal of V.N.Karazin Kharkiv

National University. Series: biology. 2014;23:107-116. Ukrainian. 8. Lopatko K., Aftandiliants Ye., Kalenska S., Tonkha O. Sposib otrymannia neionnoho koloidnoho rozchynu metaliv. Patent №38459; 12.01.2009. Ukrainian.

Taran N., Batsmanova L., Kovalenko M. et al. Impact of Metal Nanoform Colloidal Solution on the Adaptive Potential of Plants. Nanoscale Res Lett. 2016;11:89. doi:10.1186/s11671-016-1294-z.

Musiienko M. Spektrofotometrychni metody v praktytsi fiziolohii, biokhimii ta ekolohii roslyn / MM Musiienko, TV Parshykova, PS Slavnyi. K.: Fitosotsiotsentr; 2001. 200 p. Ukrainian.

Batsmanova L., Taran N. Skryninh adaptyvnoho potentsialu roslyn za pokaznykamy oksydnoho stresu. K.: TOV "Vydavnytstvo Aveha"; 2010. 79 p. Ukrainian.

Taran N., Batsmanova L., Kalenskyi V. et al. Vykorystannia nanochastok metaliv u tekhnolohiiakh vyroshchuvannia zernovykh kultur. K.: TOV "Vydavnytstvo Aveha"; 2012. Ukrainian.

Mohsenzadeh S., Moosavian S. Zinc Sulphate and Nano-Zinc Oxide Effects on Some Physiological Parameters of Rosmarinus officinalis. American Journal of Plant Sciences. 2017;8:2635-2649. doi:10.4236/ajps.2017.811178.

Khan A., Khan S., Khan M. et al. The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review. Environ Sci Pollut Res. 2015;22:13772. doi:10.1007/s11356-015-4881-0.

Eremchenko O., Kusakyna M., Luzyna E. Soderzhanye pyhmentov v rastenyiakh Lepidium sativum v uslovyiakh khlorydno-natryevoho zasolenyia y oshchelachyvanyia. Vestnyk Permskoho unyversyteta. Seryia: Byolohyia. 2014;1:30-37. Russian.

Green B., Durnford D. The chlorophyll-carotenoid proteins of oxygenic photosynthesis. Annu Rev Plant Physiol Plant Mol Biol.1996;47:685-714.

Kosyk O., Khomenko I., Batsmanova L. et al. Phenylalanine ammonia-lyase activity and anthocyanin content in different varieties of lettuce under the cadmium influence. The Ukrainian Biochemical Journal. 2017;89(2):85-91. doi:10.15407/ubj89.02.085.

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Received: 02.04.2018

Revised: 10.05.2018

Signed for publication: 10.05.2018




DOI: http://dx.doi.org/10.17721/1728_2748.2018.75.37-45

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