MOLECULAR AND GENETIC CHARACTERISTICS OF SURFACE AND NONSTRUCTURE PROTEINS OF PANDEMIC INFLUENZA VIRUSES A(H1N1)PDM09 IN 2015-2016 EPIDEMIC SEASON

O. Smutko, L. Radchenko, A. Mironenko
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; SI "Gromashevsky L.V. Institute of epidemiology and infectious diseases, NAMS of Ukraine", Kyiv, Ukraine; SI "Gromashevsky L.V. Institute of epidemiology and infectious diseases, NAMS of Ukraine", Kyiv, Ukraine

Abstract


The aim of the present study was identifying of molecular and genetic changes in hemaglutinin (HA), neuraminidase (NA) and non-structure protein (NS1) genes of pandemic influenza A(H1N1)pdm09 strains, that circulated in Ukraine during 2015-2016 epidemic season. Samples (nasopharyngeal swabs from patients) were analyzed using real-time polymerase chain reaction (RTPCR). Phylogenetic trees were constructed using MEGA 7 software. 3D structures were constructed in Chimera 1.11.2rc software.Viruses were collected in 2015-2016 season fell into genetic group 6B and in two emerging subgroups, 6B.1 and 6B.2 by gene of HA and NA. Subgroups 6B.1 and 6B.2 are defined by the following amino acid substitutions. In the NS1 protein were identified new amino acid substitutions D2E, N48S, and E125D in 2015-2016 epidemic season. Specific changes were observed in HA protein antigenic sites, but viruses saved similarity to vaccine strain. NS1 protein acquired substitution associated with increased virulence of the influenza virus.

Keywords


A(H1N1)pdm09 influenza virus, amino acid substitution, antigenic site, non-structure protein

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References


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