GENETIC CHARACTERIZATION OF INFECTIOUS BURSAL DISEASE VIRUS ISOLATES IN UKRAINE

A. Pastyria, V. Polischuk, I. Sobko
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; Center of Veterynary Diagnostics, Kyiv, Ukraine

Abstract


The objective of the investigation was to characterize infectious bursal disease viruses (IBDV) circulating in commercial poultry farms in Ukraine between 2014 and 2016. IBDV genetic material was amplified directly from bursa. The nucleotide sequence for VP2 hypervariable region of 16 IBDVs were determined by RT-PCR method, sequenced and compared to well characterised IBDV isolates worldwide. Neighbor-joining method was used for phylogenetic analyses. In result of the studyUkrainian IBDVs represented two genetic lineages: very virulent (vv) IBDVs and classical IBDV closely related to attenuated vaccine stains. The nucleotide identity among UkrainianvvIBDVs ranged between 87.2% and 99,8%. Ukrainian vvIBDV strains clustered together with very virulent strains from other counties like: United Kingdom, Egypt, China, Netherlands and Spain. In conclusion this report demonstrates the circulation of vvIBDV in commercial poultry farms in Ukraine.

Keywords


Infectious bursal disease virus, vvIBDV, VP2, RT-PCR, sequencing, phylogenetic analyses

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References


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